Functionally and structurally distinct NK cell receptor repertoires in the peripheral blood of two human donors
Functionally and structurally distinct NK cell receptor repertoires in the peripheral blood of two human donors
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DOI：
10.1016/s1074-7613(00)80393-3
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发表时间：
1997-12-01
期刊：
影响因子：
32.4
通讯作者：
Parham, P
中科院分区：
文献类型：
--
作者：
Valiante, NM;Uhrberg, M;Parham, P
KIR和CD 94：NKG 2受体的表达测定了100多个自然杀伤（NK）细胞克隆，这些细胞克隆来自两个HLA I类和KIR基因不同的献血者。超过98%的克隆被单个自体I类同种异型抑制，并且每个克隆被自体同种异型的组合抑制。抑制模式与特定特异性抑制受体的表达相关。一个供体具有KIR的三个I类配体，大多数NK细胞使用KIR作为其抑制性受体;第二个供体仅具有KIR的单一配体，大多数NK细胞使用更广泛反应性的CD 94：NKG 2a作为其抑制性受体。由于这些差异，第一供体具有杀死第二供体的细胞的NK细胞亚群，而第二供体的NK细胞普遍耐受来自第一供体的细胞。
The expression of KIR and CD94:NKG2 receptors was determined for more than 100 natural killer (NK) cell clones obtained from two blood donors who differ in their HLA class I and KIR genes. More than 98% of the clones were inhibited by individual autologous class I allotypes, and every clone was inhibited by the combination of autologous allotypes. The patterns of inhibition correlate with expression of inhibitory receptors of defined specificity. One donor possesses three class I ligands for KIR, and a majority of NK cells use KIR as their inhibitory receptor; the second donor possesses only a single ligand for KIR, and a majority of NK cells use the more broadly reactive CD94:NKG2a as their inhibitory receptor. Because of these differences, the first donor has subpopulations of NK cells that kill cells of the second donor, whereas the NK cells of the second donor are universally tolerant of cells from the first donor.