An improved method to evaluate the o-diphenol oxidase activity of soil
An improved method to evaluate the o-diphenol oxidase activity of soil
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DOI：
10.1016/s0038-0717(00)00168-1
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发表时间：
2000-11
影响因子：
9.7
通讯作者：
P. Perucci;C. Casucci;S. Dumontet
中科院分区：
文献类型：
--
作者：
P. Perucci;C. Casucci;S. Dumontet
酚类化合物是许多天然物质和合成化学品的中间转化产物，包括作物残留物、有机废物、农药和工业产品。它们是土壤微生物群产生酚酶和过氧化物酶的适宜底物。虽然测量酚类化合物的酶促氧化是土壤微生物群降解潜在顽固性有机物能力的重要指标，但执行该测量的拟议方法很少且存在问题。本文提出了一种测定全样土壤中邻二酚氧化酶活性的简便可靠的新方法。该方法是基于分光光度法测定一种红色化合物（4-（n -脯氨酸）-邻苯醌），该化合物是在脯氨酸存在下由儿茶酚酶氧化产生的。
Phenolic compounds are intermediary transformation products of many naturally occurring substances and synthetic chemicals, including crop residues, organic wastes, pesticides and industrial products. They are suitable substrates for the phenolases and peroxidases produced by the soil microflora. Although measurement of the enzymatic oxidation of phenolic compounds is an important measure of the soil microflora's capacity to degrade potentially recalcitrant organics, the proposed methods to perform this measurement are few and questionable. In this paper a new, reliable and simple method to measure the o-diphenol oxidase activity in whole soil sample is proposed. The method is based on a spectrophotometric determination of a red compound (4-(N-proline)-o-benzoquinone) developed from enzymatic oxidation of catechol in the presence of proline.