Interleukin-1β Induces Angiogenesis and Innervation in Human Intervertebral Disc Degeneration
Interleukin-1β Induces Angiogenesis and Innervation in Human Intervertebral Disc Degeneration
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DOI：
10.1002/jor.21210
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Shin, Dong Eun
中科院分区：
文献类型：
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作者：
Lee, Jae Man;Song, Ji Ye;Shin, Dong Eun
椎间盘退行性疾病（IVD）的一般特征在于基质降解增强、血管生成、神经支配和分解代谢细胞因子表达增加。在这项研究中，我们研究了炎症细胞因子IL-1 β和TNF-α对血管生成因子血管内皮生长因子（VEGF）和神经营养因子神经生长因子（NGF）和脑源性神经营养因子（BDNF）在人IVD变性中表达的影响。IL-1 β和TNF-α刺激从患者组织中分离的髓核（NP）细胞中VEGF、NGF和BDNF的基因表达。免疫组化结果显示IL-1 β和VEGF/NGF/BDNF在人IVD组织中的表达呈正相关。患者组织的RNA表达分析也确定了VEGF和血小板内皮细胞粘附分子-1（PECAM-1）之间以及NGF/BDNF和蛋白基因产物9.5（PGP9.5）之间的正相关性。我们的研究结果表明，IL-1 β在IVD变性过程中产生，刺激VEGF、NGF和BDNF的表达，导致血管生成和神经支配。(C)2010骨科研究学会。由威利期刊公司出版骨科杂志Res. 29：265-269，2011
Degenerative disorders of the intervertebral discs (IVDs) are generally characterized by enhanced matrix degradation, angiogenesis, innervation, and increased expression of catabolic cytokines. In this study, we investigated the effects of inflammatory cytokines, IL-1 beta, and TNF-alpha, on the expression of an angiogenic factor, vascular endothelial growth factor (VEGF), and neurotrophic factors, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), in human IVD degeneration. IL-1 beta and TNF-alpha stimulated the gene expression of VEGF, NGF, and BDNF in nucleus pulposus (NP) cells isolated from patient tissues. Immunohistochemical results demonstrated a positive correlation between IL-1 beta and VEGF/NGF/BDNF expression in human IVD tissues. RNA expression analysis of patient tissues also identified positive correlations between VEGF and platelet endothelial cell adhesion molecule-1 (PECAM-1) and between NGF/BDNF and protein gene product 9.5 (PGP9.5). Our findings suggest that IL-1 beta is generated during IVD degeneration, which stimulates the expression of VEGF, NGF, and BDNF, resulting in angiogenesis and innervation. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J. Orthop. Res. 29: 265-269, 2011