Characterization of two chitinase genes and one chitosanase gene encoded by chlorella virus PBCV-1
Characterization of two chitinase genes and one chitosanase gene encoded by chlorella virus PBCV-1
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DOI：
10.1006/viro.1999.9958
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发表时间：
1999-10-25
期刊：
影响因子：
3.7
通讯作者：
Van Etten, JL
中科院分区：
文献类型：
--
作者：
Sun, LW;Adams, B;Van Etten, JL
小球藻病毒PBCV-1编码两个几丁质酶基因a181/182r和a260r以及一个壳聚糖酶基因a292l。将这三个基因克隆并在大肠杆菌中表达。重组蛋白A181/182R具有内切几丁质酶活性，重组蛋白A260R同时具有内切几丁质酶和外切几丁质酶活性，重组蛋白A292L具有壳聚糖酶活性。a181/182r、a260r和a292l基因的转录在感染后30、60和60分钟开始，所有三个基因的转录持续直到细胞裂解。A181/182R、A260R和A292L蛋白首先在注射后60、90和120分钟通过蛋白质印迹检测，分别因此，a181/182r是一个早期基因，a260r和a292l是一个晚期基因。所有这三种基因都广泛存在于雷氏杆菌病毒中。系统发育分析表明，a181/182r基因的祖先条件来自烟草中发现的一个基因的最近共同祖先，而a260r基因的系谱位置不能明确解决。(C)北京：科学出版社.
Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a292l. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recombinant A292L has chitosanase activity. Transcription of a181/182r, a260r, and a 292l genes begins at 30, 60, and 60 min p.i., respectively; transcription of all three genes continues until the cells lyse. A181/182R, A260R, and A292L protein are first detected by Western blots at 60, 90, and 120 min p.i., respectively. Therefore, a181/182r is an early gene and a260r and a292l are late genes. All three genes are widespread in chlorella viruses. Phylogenetic analyses indicate that the ancestral condition of the a181/182r gene arose from the most recent common ancestor of a gene found in tobacco, whereas the genealogical position of the a260r gene could not be unambiguously resolved. (C) 1999 Academic Press.