OXYGEN-TENSION IN THE OVIDUCT AND UTERUS OF RHESUS-MONKEYS, HAMSTERS AND RABBITS
OXYGEN-TENSION IN THE OVIDUCT AND UTERUS OF RHESUS-MONKEYS, HAMSTERS AND RABBITS
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DOI：
10.1530/jrf.0.0990673
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发表时间：
1993-11-01
期刊：
影响因子：
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通讯作者：
BAVISTER, BD
中科院分区：
文献类型：
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作者：
FISCHER, B;BAVISTER, BD
用柔性极谱微电极测定了恒河猴（n = 9）、金黄地鼠（n = 21）和家兔（n = 6）在生殖周期（猴）、发情期和假孕期（仓鼠、家兔）和妊娠期（仓鼠）输卵管和子宫腔内的氧分压。在一般情况下，在每个物种的氧张力远低于大气中的O-2的一半，范围从约60毫米汞柱（8.7% O-2）的高值在兔子输卵管，兔子和仓鼠子宫，低至II毫米汞柱（1.5% O-2）在猴子子宫。氧张力没有显着变化之间的左侧和右侧的生殖道（所有物种），也没有怀孕和假孕状态之间，也没有输卵管和子宫（仓鼠）。在猴输卵管、仓鼠输卵管和子宫、兔子宫中发现了由于生殖阶段的差异。在整个月经周期中，猴子宫中的氧张力始终非常低（11-14 mm Hg）。在仓鼠和兔中，在胚泡形成和着床的正常时间，子宫内O-2显著降低，分别为37 mm Hg（5.3%O-2）和24 mm Hg（3.5%O-2）。这项研究表明，胚胎在低氧浓度下体内发育，特别是在围着床期。这些数据对研究胚胎代谢和改善体外胚胎发育具有重要意义。
Oxygen tension was measured using flexible polarographic microelectrodes within the oviductal and uterine lumen in rhesus monkeys (n = 9), golden hamsters (n = 21) and rabbits (n = 6), during the reproductive cycle (monkey), during oestrus and pseudopregnancy (hamsters, rabbits) and during pregnancy (hamsters). In general, oxygen tensions in each species were much less than half of atmospheric O-2, ranging from high values of about 60 mm Hg (8.7% O-2) in the rabbit oviduct, rabbit and hamster uterus, to as low as II mm Hg (1.5% O-2) in the monkey uterus. Oxygen tensions did not vary significantly between left and right sides of the reproductive tracts (all species), nor between pregnant and pseudopregnant states nor between oviduct and uterus (hamsters). Differences owing to reproductive stage were found in the monkey oviduct, hamster oviduct and uterus, and rabbit uterus. Oxygen tensions were consistently very low (11-14 mm Hg) in the monkey uterus throughout the menstrual cycle. In hamsters and rabbits, intrauterine O-2 decreased significantly at about the normal time of blastocyst formation and implantation, to 37 mm Hg (5.3% O-2) and 24 mm Hg (3.5% O-2), respectively. This study indicates that embryos develop in vivo under low oxygen concentrations, especially during the peri-implantation period. The data have implications for investigations of embyro metabolism and for improving embryo development in vitro.