Biohydrogen production by a novel thermotolerant photosynthetic bacterium Rhodopseudomonas pentothenatexigens strain KKU-SN1/1
Biohydrogen production by a novel thermotolerant photosynthetic bacterium Rhodopseudomonas pentothenatexigens strain KKU-SN1/1
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DOI：
10.1016/j.ijhydene.2014.07.091
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发表时间：
2014-09
影响因子：
7.2
通讯作者：
Parichat Phankhamla;Jutaporn Sawaengkaew;P. Buasri;Polson Mahakhan
中科院分区：
文献类型：
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作者：
Parichat Phankhamla;Jutaporn Sawaengkaew;P. Buasri;Polson Mahakhan
从162个环境样品中分离到39株紫色非硫细菌（PNSB）。测试了这些PNSB分离株在40 °C下产生生物氢（Bio-H2）的能力，并且注意到只有14个分离株具有这种能力，其被认为是耐热光合Bio-H2产生细菌（tt-PHPB）。在14株tt-PHPB菌株中，KKU-SN 1/1的生物产氢能力最强。采用中心组合设计对KKU-SN 1/1菌株最大产氢量的操作条件进行优化。在最佳条件下（苹果酸7.6 g/L，谷氨酸11 g/L，pH 6.7，温度40 °C），生物产氢量提高了68.28%。经气相色谱分析（GC-TCD）检测，Bio-H2的纯度为92.22%。根据形态特征和16 S rDNA序列分析，将菌株KKU-SN 1/1鉴定为Rhodopropyldomonaspentothenatexigens，同源性为99.7%。据我们所知，这是第一个关于R. pentothenatexigens KKU-SN 1/1生物产氢的报道。
Thirty-nine purple non-sulfur bacteria (PNSB) were isolated from 162 environmental samples. These isolates of PNSB were tested for their ability to produce biohydrogen (Bio-H2) at 40 °C and only 14 isolates were noted to possess such ability, which were considered as thermotolerant photosynthetic Bio-H2producing bacteria (tt-PHPB). Of 14 isolates of tt-PHPB, KKU-SN1/1 was observed to have the highest Bio-H2productivity. Central composite design was employed to optimize the operating conditions for maximal Bio-H2production by the strain KKU-SN1/1. Under optimal conditions (7.6 g/L malic acid, 11 g/L glutamic acid, pH 6.7, at 40 °C) the Bio-H2production was increased by 68.28%. The purity of Bio-H2produced was 92.22%, as suggested by gas chromatography (GC-TCD). Based on morphological characteristics and 16S rDNA sequence analysis, the strain KKU-SN1/1 was identified as Rhodopseudomonaspentothenatexigens, with 99.7% similarity. To our knowledge, this is the first report on Bio-H2production byR.pentothenatexigensKKU-SN1/1.