HUMAN PANCREATIC PHOSPHOLIPASE A(2) STIMULATES THE GROWTH OF HUMAN PANCREATIC-CANCER CELL-LINE
HUMAN PANCREATIC PHOSPHOLIPASE A(2) STIMULATES THE GROWTH OF HUMAN PANCREATIC-CANCER CELL-LINE
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DOI：
10.1016/0014-5793(95)01005-y
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发表时间：
1995-10-02
期刊：
影响因子：
3.5
通讯作者：
SUGIYAMA, M
中科院分区：
文献类型：
--
作者：
HANADA, K;KINOSHITA, E;SUGIYAMA, M
来自人胰腺的磷脂酶 A(2) (PLA(2))，称为 hPLA(2)-I，起到消化酶的作用。有趣的是，本研究表明 hPLA(2)-I 的成熟形式刺激人胰腺癌细胞系 MIAPaCa-2 的生长，而前体形式无效。来自 Laticauda semifasciata I 级分、响尾蛇毒液、紫色链霉菌和蜂毒的 PLA(2) 对 MIAPaCa-2 的生长没有增殖作用。 Scatchard图分析表明MIAPaCa-2细胞具有成熟hPLA(2)-I的特异性结合位点。平衡结合常数(K-d)和最大结合容量(B-max)分别为2.6 nM和0.4 fmol/10(6)细胞。这些结果表明成熟的hPLA(2)-I，而不是前体，可能通过特异性结合位点充当胰腺癌的生长因子。
Phospholipase A(2) (PLA(2)) from human pancreas, designated hPLA(2)-I, functions as a digestive enzyme, Interestingly, the present study demonstrated that the mature form of hPLA(2)-I stimulated the growth of a human pancreatic cancer cell line MIAPaCa-2, rvhereas the pro-form was ineffective. PLA(2)s from Laticauda semifasciata fraction I, Crotalus adamanteus venom, Streptomyces violaceoruber and bee venom, showed no proliferative effect to the growth of MIAPaCa-2. The Scatchard plot analysis revealed that the MIAPaCa-2 cell had a specific binding site for the mature hPLA(2)-I. The equilibrium binding constant (K-d) and the maximum binding capacity (B-max) were 2.6 nM and 0.4 fmol/10(6) cells, respectively. These results suggest that the mature hPLA(2)-I, but not the pro-form, may function as a growth factor of pancreas carcinoma via the specific binding site.