Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis
Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis
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DOI：
10.1073/pnas.96.17.9642
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发表时间：
1999-08-17
影响因子：
11.1
通讯作者：
Grossman, AD
中科院分区：
文献类型：
--
作者：
Levin, PA;Kurtser, IG;Grossman, AD
在细菌细胞周期中，微管样细胞分裂蛋白FtsZ聚合成环状结构，确定新生分裂部位的位置。我们已经在枯草芽孢杆菌中发现了一种FtsZ环形成的调节子，这种蛋白EzrA调节FtsZ环形成的频率和位置。Ezra的缺失导致细胞在极点和中点都有多个FtsZ环。此外，在Ezra缺失突变体中，形成环所需的FtsZ临界浓度低于野生型细胞。Ezra与细胞膜结合，并与FtsZ共同定位于新生隔区。我们认为Ezra要么与FtsZ相互作用，要么与其结合伙伴之一相互作用，以促进解聚。
During the bacterial cell cycle, the tubulin-like cell-division protein FtsZ polymerizes into a ring structure that establishes the location of the nascent division site. We have identified a regulator of FtsZ ring formation in Bacillus subtilis, This protein, EzrA, modulates the frequency and position of FtsZ ring formation. The loss of ezrA resulted in cells with multiple FtsZ rings located at polar as well as medial sites. Moreover, the critical concentration of FtsZ required for ring formation was lower in ezrA null mutants than in wild-type cells. EzrA was associated with the cell membrane and also colocalized with FtsZ to the nascent septal site. We propose that EzrA interacts either with FtsZ or with one of its binding partners to promote depolymerization.