Prevalence of human parechovirus in The Netherlands in 2000 to 2007
Prevalence of human parechovirus in The Netherlands in 2000 to 2007
复制标题
DOI：
10.1128/jcm.00168-08
复制
发表时间：
2008-09-01
影响因子：
9.4
通讯作者：
van der Avoort, Harrie
中科院分区：
文献类型：
--
作者：
van der Sanden, Sabine;de Bruin, Erwin;van der Avoort, Harrie
2004年，日本首次描述了人类3型parechovirus （HPeV3）感染，据报道，与感染HPeV1或HPeV2相比，它更常与严重疾病相关。2004年，在荷兰首次观察到HPeV3病毒感染。遗传分析显示了几种不同的谱系，提示地方性流行。我们分析了163个细胞培养分离物，这些分离物来自相同数量的患者，作为国家肠道病毒监测计划的一部分，在常规病毒学实验室进行了检测。分离株收集于2000年至2007年，无法用常规方法鉴定。通过针对5'非翻译区的逆转录- pcr检测，总共发现155株（95%）HPeV阳性，这解释了近年来肠道病毒监测中的大部分诊断缺陷。利用VP1/2A区部分基因组测序对分离株进行分型，发现55株HPeV1、2株HPeV2、89株HPeV3、1株HPeV4和8株HPeV5分离株。我们比较了隔离日期、受影响年龄组和临床图片，这些都是常规监测报告的一部分。在流行病学方面观察到明显的差异，HPeV3每隔2年发生一次，并在春夏季节发生，而HPeV1每年都有少量发生，在夏季较低。与HPeV1感染相比，HPeV3感染的儿童年龄更小，并且与发热、脑膜炎和病毒血症的关联更为明显。
Infection with human parechovirus 3 (HPeV3) was described for the first time in Japan in 2004 and reportedly is more often associated with severe disease than infection with HPeV1 or HPeV2. In 2004, infections with HPeV3 were observed for the first time in The Netherlands. Genetic analysis showed several different lineages, suggesting endemic circulation. We analyzed 163 cell culture isolates from the same number of patients tested in routine virological laboratories as part of the national enterovirus surveillance program. Isolates were collected between 2000 and 2007 and could not be characterized by routine methods. In total, 155 isolates (95%) were found positive for HPeV by a reverse transcription-PCR assay targeting the 5' untranslated region, explaining the majority of the diagnostic deficit in enterovirus surveillance for these years. Typing of the isolates by use of partial genome sequencing of the VP1/2A region revealed the presence of 55 HPeV1, 2 HPeV2, 89 HPeV3, 1 HPeV4, and 8 HPeV5 isolates. We compared isolation dates, age groups affected, and clinical pictures, which were reported as part of the routine surveillance. Clear differences in epidemiology were observed, with HPeV3 occurring at intervals of 2 years and in the spring-summer season, whereas HPeV1 was observed in small numbers throughout each year, with a low in the summer months. HPeV3 infection affected younger children than HPeV1 infection and was significantly more often associated with fever, meningitis, and viremia.