Experimental Human Infection with Neisseria gonorrhoeae
Experimental Human Infection with Neisseria gonorrhoeae
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DOI：
10.1007/978-1-4939-9496-0_25
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Duncan, Joseph A.
中科院分区：
文献类型：
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作者：
Hobbs, Marcia M.;Duncan, Joseph A.
男性志愿者实验性感染淋病奈瑟菌是安全的，并能再现自然获得性淋球菌性尿道炎的临床特征。人类模型是有用的，用于测试的重要性，推定的淋球菌毒力因子尿道感染的男性和模型提供了机会，以检查宿主的免疫反应，可以利用或改进淋球菌疫苗的开发和测试。在本章中，我们描述了N的产生、表征和储存方法。用于实验性人类挑战的淋病菌种、接种物悬液的制备和递送、监测实验性感染以及数据分析的统计考虑。
Experimental infection of male volunteers with Neisseria gonorrhoeae is safe and reproduces the clinical features of naturally acquired gonococcal urethritis. The human model is useful for testing the importance of putative gonococcal virulence factors for urethral infection in men and the model presents opportunities to examine host immune responses that may be exploited or improved in development and testing of gonococcal vaccines. In this chapter, we describe methods for production, characterization, and storage of N. gonorrhoeae stocks for experimental human challenge, preparation and delivery of inoculum suspensions, monitoring experimental infection, and statistical considerations for data analysis.