Beyond the transect: an alternative microchemical imaging method for fine scale analysis of trace elements in fish otoliths during early life.
Beyond the transect: an alternative microchemical imaging method for fine scale analysis of trace elements in fish otoliths during early life.
复制标题
DOI：
10.1016/j.scitotenv.2014.05.115
复制
发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
N. McGowan;Ashley M. Fowler;K. Parkinson;D. Bishop;K. Ganio;P. Doble;D. Booth;D. Hare
中科院分区：
文献类型：
--
作者：
N. McGowan;Ashley M. Fowler;K. Parkinson;D. Bishop;K. Ganio;P. Doble;D. Booth;D. Hare
鱼类耳石（用于平衡和定向的钙化“耳石”）的微化学分析是研究其环境历史和管理的重要工具。然而，所达到的空间分辨率往往太粗糙，无法检查生命早期发生的短期事件。目前的方法依赖于单一的点或横断面的耳石表面，这可能提供了一个有限的观点元素分布，以前没有被调查的问题。通过激光烧蚀-电感耦合等离子体质谱（LA-ICP-MS）成像，可以对早期生命中的短期事件进行微量化学分析，并具有高分辨率（< 10 μm）和元素分布的二维（2D）可视化。为了证明这种方法的潜力，我们使用8 μm的光束直径（激光能量密度为13.8 ± 3.5 J cm− 2）绘制了少量氖雀鲷（Pomacentrus coelestis）幼鱼耳石中两种关键微量元素Sr和Ba的浓度。使用Longerich等人（1996）建立的方法进行定量，该方法首次应用于生物基质的2D成像。使用Longerich的计算方法对国家标准与技术研究所（NIST）610和612（玻璃中的微量元素）进行多点非基质匹配校准，并使用基质匹配标准FEBS-1（粉末红笛鲷[Lutjanus campechanus]耳石），实现了> 97%的准确度。在耳石中实现的空间分辨率对应于幼虫期的2 ± 1天和定居后幼年期的4 ± 1天。这种方法有可能提高早期生活史事件的解释在规模对应于特定的事件。虽然图像显示Sr和Ba的梯度在整个幼虫定居区更清楚地比单一的横断面，该方法证明了样品的均匀性，整个结构，表明二维扫描没有显着的优势，线扫描。
Microchemical analysis of otolith (calcified ‘ear stones’ used for balance and orientation) of fishes is an important tool for studying their environmental history and management. However, the spatial resolution achieved is often too coarse to examine short-term events occurring in early life. Current methods rely on single points or transects across the otolith surface, which may provide a limited view of elemental distributions, a matter that has not previously been investigated. Imaging by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) permits microchemical analyses of short-term events in early life with high (< 10 μm) resolution, two-dimensional (2D) visualization of elemental distributions. To demonstrate the potential of this method, we mapped the concentrations of Sr and Ba, two key trace elements, in a small number of juvenile otoliths of neon damselfish (Pomacentrus coelestis) using an 8 μm beam diameter (laser fluence of 13.8 ± 3.5 J cm− 2). Quantification was performed using the established method by Longerich et al. (1996), which is applied to 2D imaging of a biological matrix here for the first time. Accuracy of > 97% was achieved using a multi-point non matrix-matched calibration of National Institute of Standards and Technology (NIST) 610 and 612 (trace elements in glass) using Longerich's calculation method against the matrix-matched standard FEBS-1 (powdered red snapper [Lutjanus campechanus] otolith). The spatial resolution achieved in the otolith corresponded to a time period of 2 ± 1 days during the larval phase, and 4 ± 1 days during the post-settlement juvenile phase. This method has the potential to improve interpretations of early life-history events at scales corresponding to specific events. While the images showed gradients in Sr and Ba across the larval settlement zone more clearly than single transects, the method proved sample homogeneity throughout the structure; demonstrating that 2D scanning has no significant advantage over line scans.