Cloning and characterization of full-length human ribosomal protein L15 cDNA which was overexpressed in esophageal cancer
Cloning and characterization of full-length human ribosomal protein L15 cDNA which was overexpressed in esophageal cancer
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DOI：
10.1016/s0378-1119(00)00570-9
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发表时间：
2001-01-24
期刊：
影响因子：
3.5
通讯作者：
Liu, ZH
中科院分区：
文献类型：
--
作者：
Wang, Q;Yang, CH;Liu, ZH
本研究的目的是寻找在食管癌中差异表达的基因。采用抑制性消减杂交（SSH）和反向北方高密度杂交相结合的方法，获得了一个在70%（18/26）的食管癌组织中高表达的基因，命名为EC 45。通过辐射杂交作图（RH作图）将EC 45定位于3 p12 -3p11.2。通过食管癌cDNA文库筛选，获得了EC 45全长cDNA（1987 bp）。EC 45基因编码204个氨基酸，与核糖体蛋白L15（635 bp，mRNA）在开放阅读框（ORF）上有100%的相似性，但在5'和3'非翻译区（UTR）上没有相似性。多个成人正常组织的北方印迹板显示EC 45分布在几乎正常的测试组织中。以上结果提示，编码核糖体蛋白L15的EC 45在食管癌中的高表达可能在食管癌的发生发展中起一定作用。(C)2001 Elsevier Science B. V.保留所有权利。
The aim of this investigation was trying to identify the genes differentially expressed in esophageal cancer. By combining suppression subtractive hybridization (SSH) with reverse Northern high density blots, a gene named EC45 was obtained, which dramatically overexpressed in 70% esophageal cancer (18/26). EC45 was mapped to 3p12-3p11.2 by radiation hybrid mapping (RH mapping). The putative full length EC45 cDNA (1987 bp) was identified by cDNA libraries screening of esophageal cancer. EC45 encoded 204 amino acids, and it shared a 100% similarity with ribosomal protein L15 (635 bp, mRNA) in ORF, but no similarity in 5' UTR or 3' UTR. Northern blot panel of multiple adult human normal tissues showed EC45 distributed in almost normal tissues tested. All these data suggested that EC45, encoding ribosomal protein L15 and overexpressing in esophageal cancer might play a possible role in carcinogenesis of esophagus. (C) 2001 Elsevier Science B.V. All rights reserved.