Hydrogen sulphide increases hepatic differentiation of human tooth pulp stem cells compared with human bone marrow stem cells
Hydrogen sulphide increases hepatic differentiation of human tooth pulp stem cells compared with human bone marrow stem cells
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DOI：
10.1111/iej.12262
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发表时间：
2014-12-01
影响因子：
5
通讯作者：
Haapasalo, M.
中科院分区：
文献类型：
--
作者：
Okada, M.;Ishkitiev, N.;Haapasalo, M.
目的 探讨人骨髓干细胞(hBMC)与人脱落乳牙髓干细胞(SHED)在干细胞特性、肝分化方面的差异以及硫化氢(H2S)对肝分化的影响。方法采用磁力分离CD117(+)细胞并进行肝分化。通过 qRT-PCR 表征 CD117(+) 细胞谱系的干细胞转录因子。在分化的最后9天，测试细胞暴露于0.1ngmL(-1) H2S。分化后进行白蛋白、甲胎蛋白和氨甲酰磷酸合成酶的免疫细胞化学和流式细胞术检测。还测定了尿素浓度和糖原合成。结果SHED中表达的基因也在BMC中表达。免疫荧光显示肝脏标志物的表达水平没有差异。 SHED 显示出比 hBMC 更多的阳性细胞（P
AimTo determine the differences in stem cell properties, in hepatic differentiation and in the effects of hydrogen sulphide (H2S) on hepatic differentiation between human bone marrow stem cells (hBMC) and stem cells from human exfoliated primary tooth pulp (SHED).MethodologyCD117(+) cells were magnetically separated and subjected to hepatic differentiation. CD117(+) cell lineages were characterized for transcription factors indicative of stem cells by qRT-PCR. For the last 9days of the differentiation, the test cells were exposed to 0.1ngmL(-1) H2S. Immunocytochemistry and flow cytometry of albumin, alpha-fetoprotein and carbamoyl phosphate synthetase were carried out after differentiation. Urea concentration and glycogen synthesis were also determined.ResultsGenes expressed in SHED were also expressed in BMC. No difference in expression level of hepatic markers was shown by immunofluorescence. SHED showed more positive cells than hBMC (P