Human chorionic gonadotropin and 8-bromo cyclic adenosine monophosphate promote an acute increase in cytochrome P450scc and adrenodoxin messenger RNAs in cultured human granulosa cells by a cycloheximide-insensitive mechanism.
Human chorionic gonadotropin and 8-bromo cyclic adenosine monophosphate promote an acute increase in cytochrome P450scc and adrenodoxin messenger RNAs in cultured human granulosa cells by a cycloheximide-insensitive mechanism.
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人绒毛膜促性腺激素和 8-溴环单磷酸腺苷通过放线菌酮不敏感机制促进培养的人颗粒细胞中细胞色素 P450scc 和肾上腺素信使 RNA 的急剧增加。
DOI：
10.1172/jci113149
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Strauss3rd,JF
中科院分区：
文献类型：
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作者：
Golos,TG;Miller,WL;Strauss3rd,JF
用人绒毛膜促性腺激素（hCG）或其第二信使环腺苷酸（cAMP）的类似物处理人颗粒细胞，可促进细胞色素P450侧链裂解（SCC）和肾上腺皮质激素的信使RNA（mRNA）的快速积累。在暴露于8-溴-cAMP的4小时内观察到这些mRNA的细胞含量增加两倍，并维持长达48小时。放线菌酮蛋白质合成的抑制并没有阻止hCG或8-溴-cAMP刺激的细胞色素P450 SCC或肾上腺素能氧还蛋白mRNA的积累。我们得出结论，人类颗粒细胞对hCG和cAMP类似物的反应迅速，编码类固醇生成机制的两个关键蛋白质的mRNA水平协调增加，并且这种刺激不需要合成蛋白质中间体。图片
Treatment of human granulosa cells with human chorionic gonadotropin (hCG) or an analogue of its second messenger, cyclic AMP (cAMP), promotes a rapid accumulation of the messenger RNAs (mRNAs) for cytochrome P450 side-chain cleavage (scc) and adrenodoxin. A twofold increase in the cellular content of these mRNAs was observed within 4 h of exposure to 8-bromo-cAMP, and was maintained for up to 48 h. Inhibition of protein synthesis by cycloheximide did not prevent the hCG- or 8-bromo-cAMP-stimulated accumulation of either cytochrome P450scc or adrenodoxin mRNAs. We conclude that human granulosa cells respond rapidly to hCG and cAMP analogues with a coordinate increase in levels of the mRNAs encoding two key proteins of the steroidogenic machinery, and that this stimulation does not require synthesis of a protein intermediate.Images