De Novo Adult Transcriptomes of Two European Brittle Stars: Spotlight on Opsin-Based Photoreception.
De Novo Adult Transcriptomes of Two European Brittle Stars: Spotlight on Opsin-Based Photoreception.
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DOI：
10.1371/journal.pone.0152988
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Flammang P
中科院分区：
文献类型：
--
作者：
Delroisse J;Mallefet J;Flammang P
下一代测序（NGS）技术允许获得更深入和更完整的转录组视图。对于非模式或新出现的模式海洋生物，NGS技术为快速获取遗传信息提供了一个很好的机会。本研究采用双末端Illumina HiSeqTM技术分析了两种欧洲脆性星星物种丝状蛇尾藻（Amphiura filiformis）和蜘蛛蛇尾藻（Ophiopsila aranea）臂组织的转录组。产生了大约4800万个Illumina读段，并且从A.丝状臂组织。O.在蜘蛛臂组织中，产生了约4700万个读段，并获得了123，324个总的单基因。24%的单基因来自A. filiformis与在线参考数据库中的序列有显著的匹配，而O. aranea，这一比例为23%。在这两个物种中，大约50%的预测注释unigenes与紫海胆的转录本显着相似，紫海胆是迄今为止最接近的物种，已经经历了完整的基因组测序和注释。对预测的脆性星星单基因进行了GO、COG和KEGG分析。我们把我们的分析集中在参与光感知的光转导因子上。首先，在棘皮动物的视蛋白中鉴定了两个新的视蛋白。蜘蛛：一种横纹肌视蛋白（与脊椎动物黑视蛋白同源）和一种RGR视蛋白。RGR-视蛋白被认为参与视网膜再生，而r-视蛋白被怀疑在视觉样行为中发挥作用。其次，潜在的光转导演员被确定在两个转录组使用苍蝇（横纹肌）和哺乳动物（纤毛）的经典光转导途径作为参考。最后，研究了蜘蛛网蛛对单色光的敏感性，以补充现有的蜘蛛网蛛对单色光的敏感性数据。丝状的结果表明，在O.蜘蛛可能解释这两个物种之间趋光行为的差异。结果证实了（i）这些脆性恒星使用视蛋白感光的能力，（ii）建议共同出现的横纹肌和睫状光感受器，和（iii）强调光感知的复杂性，在这个棘皮类。
Next generation sequencing (NGS) technology allows to obtain a deeper and more complete view of transcriptomes. For non-model or emerging model marine organisms, NGS technologies offer a great opportunity for rapid access to genetic information. In this study, paired-end Illumina HiSeqTM technology has been employed to analyse transcriptomes from the arm tissues of two European brittle star species, Amphiura filiformis and Ophiopsila aranea. About 48 million Illumina reads were generated and 136,387 total unigenes were predicted from A. filiformis arm tissues. For O. aranea arm tissues, about 47 million reads were generated and 123,324 total unigenes were obtained. Twenty-four percent of the total unigenes from A. filiformis show significant matches with sequences present in reference online databases, whereas, for O. aranea, this percentage amounts to 23%. In both species, around 50% of the predicted annotated unigenes were significantly similar to transcripts from the purple sea urchin, the closest species to date that has undergone complete genome sequencing and annotation. GO, COG and KEGG analyses were performed on predicted brittle star unigenes. We focused our analyses on the phototransduction actors involved in light perception. Firstly, two new echinoderm opsins were identified in O. aranea: one rhabdomeric opsin (homologous to vertebrate melanopsin) and one RGR opsin. The RGR-opsin is supposed to be involved in retinal regeneration while the r-opsin is suspected to play a role in visual-like behaviour. Secondly, potential phototransduction actors were identified in both transcriptomes using the fly (rhabdomeric) and mammal (ciliary) classical phototransduction pathways as references. Finally, the sensitivity of O.aranea to monochromatic light was investigated to complement data available for A. filiformis. The presence of microlens-like structures at the surface of dorsal arm plate of O. aranea could potentially explain phototactic behaviour differences between the two species. The results confirm (i) the ability of these brittle stars to perceive light using opsin-based photoreception, (ii) suggest the co-occurrence of both rhabdomeric and ciliary photoreceptors, and (iii) emphasise the complexity of light perception in this echinoderm class.