Live Imaging of Axonal Transport in the Adult Drosophila Central Nervous System.
Live Imaging of Axonal Transport in the Adult Drosophila Central Nervous System.
复制标题
成年果蝇中枢神经系统轴突运输的实时成像。
DOI：
10.1007/978-1-0716-1990-2_22
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Godenschwege,TanjaA
中科院分区：
文献类型：
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作者：
Robinson,Wayne;Godenschwege,TanjaA
轴突的实时成像允许确定蛋白质或细胞器的运动性和方向性。在果蝇中，轴突运输主要在外周神经元中进行，例如幼虫的运动神经元和成虫翅膀的感觉神经元。由于外周神经元和中枢神经系统（CNS）神经元是固有的不同，我们提供了一种方法，使用直立或倒置显微镜的宫颈结缔组织中的CNS神经元的轴突运输的实时图像。该方法包括将整个CNS解剖并安装在玻璃底培养皿中，该培养皿适用于宫颈结缔组织中几乎任何轴突的成像。在这里，我们展示了一个同时成像的两个巨大的纤维轴突，这是苍蝇的逃逸反应电路的一部分，并由于其大直径提供出色的分辨率的例子。
Live imaging of axons allows for the determination of motility and directionality of proteins or organelles. InDrosophila, axonal transport has been predominantly characterized in peripheral neurons, such as larval motor neurons and sensory neurons of the adult wing. As peripheral neurons and central nervous system (CNS) neurons are inherently different, we provide a method to live-image axonal transport of CNS neurons in the cervical connective using an upright or inverted microscope. The method involves dissecting and mounting an entire CNS in a glass bottom petri dish, which is suitable for imaging of nearly any axon in cervical connective. Here, we show an example for simultaneous imaging of both giant fiber axons, which are part of the fly’s escape response circuitry, and due to their large diameter provide outstanding resolution.