A High-Resolution Opto-Electrophysiology System With a Miniature Integrated Headstage.
A High-Resolution Opto-Electrophysiology System With a Miniature Integrated Headstage.
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DOI：
10.1109/tbcas.2018.2852267
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发表时间：
2018-07-16
影响因子：
5.1
通讯作者：
Yoon E
中科院分区：
文献类型：
--
作者：
Mendrela AE;Kim K;English D;McKenzie S;Seymour JP;Buzsaki G;Yoon E
本研究提出了一种完全集成的小尺寸（1.9 g）神经接口系统，包括一个μLED硅光电极（12 μ LED和32个记录位点，采用4柄结构）、一个Intan 32通道记录芯片和一个用于控制12 μ LED的定制光学刺激芯片。通过定制LED驱动器ASIC实现辐射通量分辨率约为68.5 nW的高分辨率光学刺激，可单独控制多达48个通道，电流精度为1 μA，最大电流为1.024 mA，更新速率> 10 kHz。记录由Intan®的现成32通道数字化前端ASIC执行。设计了两个紧凑的定制接口PCB，用于将云台与PC连接。原型系统展示了精确的电流产生、足够的光辐射通量产生（e> 0.16 μW）以及μ LED的快速开启（trise < 10 μs）。单个动物体内实验验证了头台精确调节单个神经元活动和独立调节邻近光电极柄附近的单独神经元群体的活动的能力。
This work presents a fully integrated neural interface system in a small form factor (1.9 g), consisting of a μLED silicon optoelectrode (12 μLEDs and 32 recording sites in a 4-shank configuration), an Intan 32-channel recording chip, and a custom optical stimulation chip for controlling 12 μLEDs. High-resolution optical stimulation with approximately 68.5 nW radiant flux resolution is achieved by a custom LED driver ASIC, which enables individual control of up to 48 channels with a current precision of 1 μA, a maximum current of 1.024 mA, and an update rate of > 10 kHz. Recording is performed by an off-the-shelf 32-channel digitizing front-end ASIC from Intan®. Two compact custom interface PCBs were designed to link the headstage with a PC. The prototype system demonstrates precise current generation, sufficient optical radiant flux generation (𝚽e > 0.16 μW), and fast turn-on of μLEDs (trise < 10 μs). Single animal in vivo experiments validated the headstage’s capability to precisely modulate single neuronal activity and independently modulate activities of separate neuronal populations near neighboring optoelectrode shanks.