Stereoselective reduction of ethyl 4-chloro acetoacetate with recombinant Pichia pastoris
Stereoselective reduction of ethyl 4-chloro acetoacetate with recombinant Pichia pastoris
复制标题
DOI：
10.1016/j.tetasy.2004.09.021
复制
发表时间：
2004-11-15
影响因子：
--
通讯作者：
Weuster-Botz, D
中科院分区：
文献类型：
--
作者：
Engelking, H;Pfaller, R;Weuster-Botz, D
共表达木兰假丝酵母羰基还原酶和枯草芽孢杆菌葡萄糖脱氢酶的重组毕赤酵母用于立体选择性还原4-氯乙酰乙酸乙酯1为（S）-4-氯-3-羟基丁酸酯2。以乙酸正丁酯为有机溶剂，在两相反应体系中进行生物还原。通过连续进料细胞悬浮液和酮酯1，实现了350 mM 1的完全转化，最终产率为91%，对映体过量为95%。(C)2004 Elsevier Ltd.保留所有权利。
A recombinant strain of Pichia pastoris coexpressing the carbonyl reductase of Candida magnoliae and the glucose dehydrogenase of Bacillus subtilis was used for the stereoselective reduction of ethyl 4-chloro acetoacetate 1 to (S)-4-chloro-3-hydroxybutanoate 2. The bioreduction was performed in a two-phase reaction system with n-butyl acetate as organic solvent. Complete conversion of 350 mM 1 with a final yield of 91% and an enantiomeric excess of 95% was achieved by continuous feeding of cell suspension and ketoester 1. (C) 2004 Elsevier Ltd. All rights reserved.