Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.
Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.
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连接的共表达37或连接连接与连接蛋白43的共表达有选择地影响细胞间分子转移。
DOI：
10.1007/s00232-012-9444-4
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发表时间：
2012-06
影响因子：
2.4
通讯作者：
Beyer, Eric C.
中科院分区：
文献类型：
--
作者：
Gemel, Joanna;Nelson, Tasha K.;Burt, Janis M.;Beyer, Eric C.
许多组织表达多种间隙连接蛋白或连接蛋白（Cx）;例如，Cx43、Cx40和Cx 37在血管细胞中共表达。本研究旨在阐明Cx40或Cx 37与Cx43以不同比例共表达的后果。EcR-293细胞（其内源性产生Cx43）用编码Cx 37或Cx40的蜕皮激素诱导型质粒转染。免疫印迹显示，在维持恒定水平的Cx43的同时，Ponasterone剂量依赖性诱导Cx 37或Cx40。共表达的连接蛋白共定位于附着膜。双全细胞膜片钳记录显示，在用0、0.5或4 μM的ponasterone处理的细胞中，总连接电导没有显著变化;然而，它们确实显示了与诱导的连接蛋白表达一致的单一通道大小的多样性。在诱导表达Cx40或Cx 37的细胞中，显微注射荧光黄的细胞间转移减少，但NBD-TMA（2-（4-硝基-2，1，3-苯并恶二醇-7-基）[氨乙基]三甲基铵）的转移不受影响。在含有未诱导的EcR细胞与诱导共表达Cx 37或Cx40的细胞的共培养物中，仅在单独表达Cx43的细胞之间观察到荧光黄转移。这些数据表明，在表达Cx43的细胞中诱导Cx 37或Cx40的表达可以选择性地改变某些分子的细胞间交换，而不影响其他分子的转移。
Many tissues express multiple gap junction proteins, or connexins (Cx); for example, Cx43, Cx40, and Cx37 are coexpressed in vascular cells. This study was undertaken to elucidate the consequences of coexpression of Cx40 or Cx37 with Cx43 at different ratios. EcR-293 cells (which endogenously produce Cx43) were transfected with ecdysone-inducible plasmids encoding Cx37 or Cx40. Immmunoblotting showed a ponasterone dose-dependent induction of Cx37 or Cx40 while constant levels of Cx43 were maintained. The coexpressed connexins colocalized at appositional membranes. Double whole-cell patch clamp recordings showed no significant change in total junctional conductances in cells treated with 0, 0.5, or 4 µM ponasterone; however, they did show a diversity of unitary channel sizes consistent with the induced connexin expression. In cells with induced expression of either Cx40 or Cx37, intercellular transfer of microinjected Lucifer yellow was reduced, but transfer of NBD-TMA (2-(4-nitro- 2,1,3-benzoxadiol-7-yl)[aminoethyl]trimethylammonium) was not affected. In cocultures containing uninduced EcR cells together with cells induced to coexpress Cx37 or Cx40, Lucifer yellow transfer was observed only between the cells expressing Cx43 alone. These data show that induced expression of either Cx37 or Cx40 in Cx43- expressing cells can selectively alter the intercellular exchange of some molecules without affecting the transfer of others.
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影响因子：
5.5
作者：
Cottrell, GT;Wu, Y;Burt, JM
通讯作者：
Burt, JM
影响因子：
4
作者：
Good, Miranda E.;Nelson, Tasha K.;Burt, Janis M.
通讯作者：
Burt, Janis M.
影响因子：
5
作者：
Lin, Xianming;Gemel, Joanna;Glass, Aaron;Zemlin, Christian W.;Beyer, Eric C.;Veenstra, Richard D.
通讯作者：
Veenstra, Richard D.
影响因子：
20.1
作者：
BEBLO, DA;WANG, HZ;VEENSTRA, RD
通讯作者：
VEENSTRA, RD
影响因子：
--
作者：
Burt, JM;Steele, TD
通讯作者：
Steele, TD