Differential Effects of Actinomycin D and Puromycin on the Release of Interferon induced by Double Stranded RNA
Differential Effects of Actinomycin D and Puromycin on the Release of Interferon induced by Double Stranded RNA
复制标题
放线菌素 D 和嘌呤霉素对双链 RNA 诱导的干扰素释放的不同影响
作者：
J. Vilček;T. Rossman;F. Varacalli
用合成的双链RNA 1诱导干扰素的产生被放线菌素D2，3抑制。然而，Finkelstein等4报道，抑制双链RNA诱导的干扰素产生所需的放线菌素D浓度比抑制病毒诱导的干扰素合成所需的放线菌素D浓度高得多。此外，在完整生物体5或细胞培养物4中用双链RNA处理后，干扰素的释放不会受到蛋白质合成抑制剂的抑制，而在相同条件下，病毒诱导的干扰素合成会受到抑制。
THE induction of interferon production with synthetic double stranded RNA1 is suppressed by actinomycin D2,3. Finkelstein et al.4, however, reported that considerably higher concentrations of actinomycin D were required for the suppression of interferon production induced by double stranded RNA than for the inhibition of viral-induced interferon synthesis. Moreover, the release of interferon after treatment with double stranded RNA in the intact organism5 or in cell cultures4 was not suppressed by inhibitors of protein synthesis, while viral-induced interferon synthesis was inhibited in the same conditions.