Role of the Nuclear Transport Factor p10 in Nuclear Import
Role of the Nuclear Transport Factor p10 in Nuclear Import
复制标题
核运输因子 p10 在核进口中的作用
DOI：
10.1126/science.272.5258.120
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发表时间：
1996
期刊：
影响因子：
56.9
通讯作者：
G. Blobel
中科院分区：
文献类型：
--
作者：
U. Nehrbass;G. Blobel
核输入因子p10是从酿酒酵母中克隆的，并被发现是必需的。蛋白质p10可以直接结合到几个含有肽重复的核孔蛋白。它还以鸟苷二磷酸（GDP）结合形式与鸟苷三磷酸酶（GTdR）Ran结合，并与核转运蛋白β结合。装配的核转运蛋白异源二聚体上固定的核孔蛋白产生的p10和Ran-GDP的合作结合。将GTP添加到该五聚体复合物中导致核粒转运蛋白α解离，推测是通过从Ran-GDP原位形成Ran-GTP。因此，p10似乎协调RAN依赖的关联和解离反应核进口的基础。
The nuclear import factor p10 was cloned from Saccharomyces cerevisiae and found to be essential. The protein p10 can bind directly to several peptide repeat-containing nucleoporins. It also binds to the guanosine triphosphatase (GTPase) Ran in its guanosine diphosphate (GDP)-bound form and to karyopherin β. Assembly of the karyopherin heterodimer on immobilized nucleoporin yielded cooperative binding of p10 and Ran-GDP. Addition of GTP to this pentameric complex led to dissociation of karyopherin α, presumably via in situ formation of Ran-GTP from Ran-GDP. Thus, p10 appears to coordinate the Ran-dependent association and dissociation reactions underlying nuclear import.