Tissue culture for xylem differentiation with Arabidopsis leaves.
Tissue culture for xylem differentiation with Arabidopsis leaves.
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拟南芥叶木质部分化的组织培养。
DOI：
10.1007/978-1-4939-6722-3_5
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
H*.
中科院分区：
文献类型：
--
作者：
Saito;M.;Nurani;A.M.;Kondo;Y*. and Fukuda;H*.
近年来，培养体系与分子生物学方法相结合，发现了调控血管分化的各种关键因子，为血管生物学的发展做出了巨大贡献。最近，我们建立了一个新的培养系统的木质部细胞分化的模式植物拟南芥（Arabidopsis thaliana），其中异位木质部细胞可以诱导整个叶盘在3-4天。在这里，我们描述了从样品制备到血管细胞诱导培养的培养系统的详细程序。
Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plantArabidopsis thaliana(Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3–4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.