Composition of the bacterial flora in tonsilloliths
Composition of the bacterial flora in tonsilloliths
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DOI：
10.1016/j.micinf.2006.04.023
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发表时间：
2006-08-01
影响因子：
5.8
通讯作者：
Watanabe, Tatsuo
中科院分区：
文献类型：
--
作者：
Tsuneishi, Midori;Yamamoto, Tatsuo;Watanabe, Tatsuo
扁桃体结石是口腔恶臭的潜在原因。在这项研究中，微生物的配置文件和扁桃体的组成，确定使用文化独立的分子方法和扫描电子显微镜。从6例扁桃体中提取16SrDNA，经PCR扩增后克隆到大肠杆菌中。克隆插入片段的约600个碱基的部分16S rDNA序列用于通过与已知物种的序列比较来确定物种同一性。用扫描电镜分析扁桃体结石表面和内部细菌的特征。扁桃体中检出的厌氧细菌属于真杆菌属、梭杆菌属、巨球菌属、卟啉单胞菌属、普雷沃氏菌属、月单胞菌属和坦纳氏菌属，所有这些似乎都与挥发性硫化合物的产生有关。电子显微镜检查发现扁桃体表面有球菌和杆状物，扁桃体内部以杆状物为主。这些结果支持扁桃体结石作为口腔恶臭的起源。(c)2006年，Elsevier SAS。All rights reserved.
Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera Eubacterium, Fusobacterium, Megasphaera, Porphyromonas, Prevotella, Selenomonas and Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor. (c) 2006 Elsevier SAS. All rights reserved.