Establishment of Orthotopic Liver Tumors by Surgical Intrahepatic Tumor Injection in Mice with Underlying Non-Alcoholic Fatty Liver Disease
Establishment of Orthotopic Liver Tumors by Surgical Intrahepatic Tumor Injection in Mice with Underlying Non-Alcoholic Fatty Liver Disease
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DOI：
10.3390/mps1020021
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发表时间：
2018-06-01
影响因子：
2.4
通讯作者：
Greten, Tim F.
中科院分区：
文献类型：
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作者：
Brown, Zachary J.;Heinrich, Bernd;Greten, Tim F.
非酒精性脂肪性肝病（NAFLD）及其晚期形式非酒精性脂肪性肝炎（NASH）的患病率正在增加，因此其对肝细胞癌发展的贡献也在增加。NAFLD已被证明会影响免疫肿瘤微环境。因此，在NAFLD背景下开发临床前小鼠模型越来越重要。在这里，我们描述了一种小鼠模型，旨在概括NAFLD的发现，然后通过肝内肿瘤注射快速诱导原位肝肿瘤。此外，我们利用生物发光成像来监测肿瘤生长和对治疗的反应。一个主要肿瘤结节的发展允许肿瘤和肝组织的精确分离。这对于免疫治疗研究是有用的，因为来自肿瘤和周围肝组织的单核细胞可以单独分析。
The prevalence of non-alcoholic fatty liver disease (NAFLD) and its advanced form, nonalcoholic steatohepatitis (NASH), is increasing, and as such its contribution to the development of hepatocellular carcinoma is also rising. NAFLD has been shown to influence the immune tumor microenvironment. Therefore, development of pre-clinical mouse models in the context of NAFLD are increasingly important. Here, we describe a mouse model designed to recapitulate the findings of NAFLD followed by rapid induction of orthotopic liver tumors with intrahepatic tumor injection. Additionally, we utilized bioluminescent imaging to monitor tumor growth and response to therapy. The development of one dominant tumor nodule allows precise separation of tumor and liver tissue. This is useful for immunotherapy studies as mononuclear cells from the tumor and the surrounding liver tissue can be analyzed separately.