c-Jun is essential for organization of the epidermal leading edge
c-Jun is essential for organization of the epidermal leading edge
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DOI：
10.1016/s1534-5807(03)00159-x
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发表时间：
2003-06-01
影响因子：
11.8
通讯作者：
Johnson, RS
中科院分区：
文献类型：
--
作者：
Li, GC;Gustafson-Brown, C;Johnson, RS
上皮层的迁移需要位于薄片前缘的细胞的特定和协调的组织。在表皮中有条件地删除c-jun原癌基因的小鼠将以预期的频率出生，但会睁开眼睛，表皮伤口愈合过程中存在缺陷。缺乏c-jun的角质形成细胞在Scratch实验的边界不能在培养中正常迁移或伸长。体外和体内的组织学分析表明，创面前沿的EGF受体不能被激活，我们证明，这可以通过补充条件培养液或EGF受体配体HB-EGF来挽救。C-Jun的缺失阻止了EGF诱导的HB-EGF的表达，表明c-Jun通过控制EGF受体自分泌环来控制表皮前沿的形成。
The migration of epithelial layers requires specific and coordinated organization of the cells at the leading edge of the sheet. Mice that are conditionally deleted for the c-jun protooncogene in epidermis are born at expected frequencies, but with open eyes and with defects in epidermal wound healing. Keratinocytes lacking c-Jun are unable to migrate or elongate properly in culture at the border of scratch assays. Histological analyses in vitro and in vivo demonstrate an inability to activate EGF receptor at the leading edge of wounds, and we demonstrate that this can be rescued by supplementation with conditioned medium or the EGF receptor ligand HB-EGF. Lack of c-Jun prevents EGF-induced expression of HB-EGF, indicating that c-jun controls formation of the epidermal leading edge through its control of an EGF receptor autocrine loop.