Novel role for cystic fibrosis transmembrane conductance regulator in alveolar fluid clearance in lipopolysaccharide-induced acute lung injury in mice
Novel role for cystic fibrosis transmembrane conductance regulator in alveolar fluid clearance in lipopolysaccharide-induced acute lung injury in mice
复制标题
囊性纤维化跨膜电导调节剂在脂多糖诱导的小鼠急性肺损伤中肺泡液清除中的新作用
DOI：
10.1111/resp.12114
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发表时间：
2013-08-01
期刊：
影响因子：
6.9
通讯作者：
Jiang, Jinjun
中科院分区：
文献类型：
--
作者：
Jin, Weizhong;Song, Yuanlin;Jiang, Jinjun
Background and objective: Alveolar fluid clearance (AFC) is important for the resolution of acute lung injury (ALI). The role of cystic fibrosis transmembrane conductance regulator (CFTR) in AFC has not been entirely elucidated in animal models of ALI. The aim of this study was to investigate the role of CFTR and its mechanisms in AFC in normal and ALI mice.Methods: Seventy mice were randomly divided into 14 groups and ALI was established by intratracheal instillation of lipopolysaccharide (LPS). After 48 h, CFTR activator CFTRact-16 or inhibitor CFinh-172 with or without beta-agonist was instillated intratracheally and AFC was measured with radioisotopic tracer.Results: Although there was no effects of CFTRact-16 on AFC in mice with or without isoproterenol, CFinh-172 markedly decreased isoproterenol-stimulated AFC in both normal (P < 0.01) and LPS-induced ALI mice (P < 0.01) and there was significantly decreased basal AFC in ALI mice (P < 0.05).Conclusions: These results provide direct functional evidence for CFTR in cAMP-mediated AFC in both normal and ALI mice.
