INTRAGENIC CIS-ACTING ART GENE-RESPONSIVE SEQUENCES OF THE HUMAN IMMUNODEFICIENCY VIRUS
INTRAGENIC CIS-ACTING ART GENE-RESPONSIVE SEQUENCES OF THE HUMAN IMMUNODEFICIENCY VIRUS
复制标题
DOI：
10.1073/pnas.85.7.2071
复制
发表时间：
1988-04-01
影响因子：
11.1
通讯作者：
HASELTINE, WA
中科院分区：
文献类型：
--
作者：
ROSEN, CA;TERWILLIGER, E;HASELTINE, WA
人类免疫缺陷病毒的art基因产物是病毒粒子衣壳和包膜基因蛋白的表达所必需的。这里提出的实验表明，位于包膜基因编码区内的序列对异源基因的表达产生负面影响，并且这些序列的负面影响可以通过本领域的基因产物减轻。 env基因中的该区域含有抑制基因表达的负调控序列，以及本领域基因产物依赖性抑制缓解所必需的序列。这些实验定义了调节系统的顺式和反式作用组件，该系统允许人类免疫缺陷病毒病毒粒子结构和调节蛋白的差异表达。
The art gene product of the human immunodeficiency virus is required for the expression of virion capsid and envelope gene proteins. The experiments presented here show that sequences located within the coding region of the envelope gene exert a negative effect on the expression of heterologous genes and that the negative effect of these sequences can be relieved by the art gene product. This region in the env gene contains negative regulatory sequences that inhibit gene expression, as well as a sequence necesssary for the art gene product-dependent relief of repression. The experiments define the cis- and trans-acting components of a regulatory system that permits differential expression of human immunodeficiency virus virion structural and regulatory proteins.