Expression and regulation of tumor suppressor gene maspin in breast cancer.
Expression and regulation of tumor suppressor gene maspin in breast cancer.
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DOI：
10.3816/cbc.2002.n.032
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发表时间：
2002-10
影响因子：
3.1
通讯作者：
N. Maass;K. Nagasaki;Martin Ziebart;C. Mundhenke;W. Jonat
中科院分区：
文献类型：
--
作者：
N. Maass;K. Nagasaki;Martin Ziebart;C. Mundhenke;W. Jonat
Maspin（乳腺丝氨酸蛋白酶）是一种丝氨酸蛋白酶抑制剂，在乳腺癌中具有肿瘤抑制活性。Maspin最初是通过减法杂交在正常乳腺上皮细胞中发现的，但在肿瘤进展过程中其表达减少。随着恶性肿瘤的增加，maspin基因表达的丧失是通过转录调控的。已知Maspin参与侵袭和转移，与p53肿瘤抑制通路相互作用，并作为血管生成抑制剂。正常乳腺组织和乳腺癌组织中maspin的免疫组织化学分析和逆转录聚合酶链反应表明，在导管原位癌到浸润性癌再到淋巴结转移的过程中，maspin的表达是逐步降低的。乳腺癌中缺乏maspin表达似乎与较短的无病生存期有关，并支持maspin作为肿瘤侵袭性和转移潜力指标的功能。massin基因调控的新研究为massin在肿瘤细胞中可能的再表达提供了证据。maspin作为肿瘤抑制基因参与肿瘤侵袭、转移和血管生成的功能可能并不局限于乳腺癌。
Maspin (mammary serpin) is an inhibitor of serine proteases with tumor suppressor activity in breast cancer. Maspin was originally identified by subtractive hybridization in normal breast epithelial cells, but its expression decreased during tumor progression. The loss of maspin gene expression with increasing malignancy is by transcriptional regulation. Maspin is known to be involved in invasion and metastasis, interact with the p53 tumor suppressor pathway, and act as an inhibitor of angiogenesis. The immunohistochemical analysis and reverse-transcription polymerase chain reaction of maspin in normal human breast tissue and breast carcinoma indicated a stepwise reduction of maspin expression during the progression from ductal carcinoma in situ to invasive carcinoma to lymph node metastasis. The lack of maspin expression in breast cancer seems to be associated with a short disease-free survival and supports maspin's function as an indicator for tumor aggressiveness and metastatic potential. New studies on the gene regulation of maspin provide evidence for promising potential of possible re-expression of maspin in tumor cells. The function of maspin as a tumor suppressor gene involved in tumor invasion, metastasis, and angiogenesis may not be limited to breast cancer.