Effect of combined application of bFGF and inorganic polyphosphate on bioactivities of osteoblasts and initial bone regeneration
Effect of combined application of bFGF and inorganic polyphosphate on bioactivities of osteoblasts and initial bone regeneration
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DOI：
10.1016/j.actbio.2009.01.034
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发表时间：
2009-06-01
影响因子：
9.7
通讯作者：
Akagawa, Y.
中科院分区：
文献类型：
--
作者：
Yuan, Q.;Kubo, T.;Akagawa, Y.
碱性成纤维细胞生长因子（bFGF）和无机聚磷酸盐（poly(P)）已被认为是增强骨再生的治疗剂。研究还表明，poly(P) 可以增强 bFGF 的促有丝分裂活性。本研究的目的是评估 bFGF 和 Poly(P) 对成骨细胞生物活性和体外和体内初始骨再生的联合影响。 MC3T3-E1细胞用bFGF、poly(P)或bFGF+poly(P)处理，然后进行细胞增殖测定、碱性磷酸酶(ALP)活性测定、实时定量逆转录聚合酶链反应和茜素S红染色。在一项体内研究中，制备了 bFGF-、poly(P)- 和 bFGF+poly(P)- 修饰的互连多孔羟基磷灰石 (IPHA) 复合物，并将其放入兔子的股骨中，以从组织学和组织形态计量学角度评估新骨形成。在第 5 天和第 7 天，用 bFGF+poly(P) 处理的细胞中观察到细胞增殖的增强程度最高。与未处理的细胞相比，用 bFGF+poly(P) 处理的细胞在第 5 天和第 10 天也表现出 ALP 活性增加，骨钙素和骨桥蛋白 mRNA 水平上调，并且钙化增强。在体内，bFGF+poly(P)-修饰的IPHA复合物中观察到最高的骨形成率。这项研究表明，bFGF 和聚 (P) 的共同应用可以通过调节细胞增殖和矿化过程来增强骨形成。预计bFGF与poly(P)的联合应用可为临床提供骨再生的新方法。 (c) 2009 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Basic fibroblast growth factor (bFGF) and inorganic polyphosphate (poly(P)) have been recognized as therapeutic agents that enhance bone regeneration. It has also been shown that poly(P) may enhance the mitogenic activity of bFGF. The purpose of this study is to evaluate the combined effect of bFGF and poly(P) on bioactivities of osteoblasts and initial bone regeneration in vitro and in vivo. MC3T3-E1 cells were treated with bFGF, poly(P) or bFGF+poly(P), then subjected to cell proliferation assay, alkaline phosphatase (ALP) activity measurement, quantitative real-time reverse transcription-polymerase chain reaction and Alizarin S Red staining. In an in vivo study, bFGF-, poly(P)- and bFGF+poly(P)-modified interconnected porous hydroxyapatite (IPHA) complexes were fabricated, and placed into the femurs of rabbits to evaluate new bone formation histologically and histomorphometrically. The highest enhancement of cell proliferation were observed in those treated with bFGF+poly(P) on days 5 and 7. Cells treated with bFGF+poly(P) also exhibited increased ALP activity on days 5 and 10, up-regulated mRNA levels of osteocalcin and osteopontin, and enhanced calcification when compared to the non-treated cells. In vivo, the highest bone formation ratio was observed in bFGF+poly(P)-modified IPHA complexes. This study indicated that co-application of bFGF and poly(P) may provide enhanced bone formation by modulating cell proliferation and the mineralization process. It is anticipated that a combined application of bFGF and poly(P) can provide a novel method for bone regeneration in clinical use. (c) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.