INDUCTION OF GERM-LINE IMMUNOGLOBULIN HEAVY-CHAIN TRANSCRIPTS BY MITOGENS AND INTERLEUKINS PRIOR TO SWITCH RECOMBINATION
INDUCTION OF GERM-LINE IMMUNOGLOBULIN HEAVY-CHAIN TRANSCRIPTS BY MITOGENS AND INTERLEUKINS PRIOR TO SWITCH RECOMBINATION
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DOI：
10.1002/eji.1830200520
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发表时间：
1990-05-01
影响因子：
5.4
通讯作者：
STAVNEZER, J
中科院分区：
文献类型：
--
作者：
SEVERINSON, E;FERNANDEZ, C;STAVNEZER, J
最近有人推测，免疫球蛋白类转换之前是从未重排的重链基因转录。在本报告中，我们研究了未重排的C.Gamma.3、C.Gamma.1、C.Gamma.2b、C.Gamma.2a、C.epsilon的RNA转录条件。和C.Alpha。正常脾细胞中的基因由有丝分裂原和/或白介素4、白介素5和干扰素-γ诱导。脂多糖(LPS)加IL4诱导生殖系γ-1和-epsilon。成绩单。内毒素诱导γ2b和γ3转录本，高剂量IL 4抑制这些转录本。干扰素--伽马。诱导低水平的生殖系γ2a转录本，并深刻抑制γ1和epsilon。IL-5单独或与IL-4和/或内毒素联合诱导不能诱导生殖系α。成绩单。但不表达IgG3的部分免疫缺陷小鼠CBA/N和C3H/HeJ的脾细胞可被多克隆激活剂诱导表达生殖系γ3和γ2b转录本。这些数据表明，配体诱导/抑制特定未重排重链基因转录的能力是其诱导转换为相应Ig同型的能力的良好指标。然而，也很清楚的是，可以在其他级别执行切换控制。
It has recently been postulated that immunoglobulin class switching is preceded by transcription from unrearranged heavy chain genes. In this report, we have investigated the conditions under which RNA transcribed from unrearranged C.gamma.3, C.gamma.1, C.gamma.2b, C.gamma.2a, C.epsilon. and C.alpha. genes are induced in normal spleen cells by mitogens and/or interleukin (IL) 4, IL 5 and interferon-.gamma.. Lipopolysaccharide (LPS) plus IL4 induced germ-line .gamma.1 and .epsilon. transcripts. LPS induced .gamma.2b and .gamma.3 transcripts and high doses of IL 4 suppressed these LPS-induced transcripts. Interferon-.gamma. induced low levels of germ-line .gamma.2a transcripts and profoundly suppressed the .gamma.1 and .epsilon. transcripts induced by LPS and IL 4. IL 5 alone or in combination with IL 4 and/or LPS did not induce germ-line .alpha. transcripts. Spleen cells of the partially immunodeficient mice CBA/N and C3H/HeJ, which do not express IgG3 could be induced, however, by polyclonal activators to express germ-line .gamma.3 and .gamma.2b transcripts. The data indicate that the capacity of a ligand to induce/suppress transcription of a particular unrearranged heavy chain gene is a good indicator of its capacity to induce switching to the corresponding Ig isotype. However, it is also clear that control of switching can be carried out at other levels.