Studies on Two Closely Related Species of Octocorallians: Biochemical and Molecular Characteristics of the Organic Matrices of Endoskeletal Sclerites
Studies on Two Closely Related Species of Octocorallians: Biochemical and Molecular Characteristics of the Organic Matrices of Endoskeletal Sclerites
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八珊瑚类两个密切相关物种的研究：内骨骼骨片有机基质的生化和分子特征
DOI：
10.1007/s10126-005-6150-6
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发表时间：
2006
影响因子：
3
通讯作者：
T. Uehara
中科院分区：
文献类型：
--
作者：
Md. Mizanur Rahman;Y. Isa;T. Uehara
两种珊瑚，Lobophytum crassum和Sinularia polydactyla，彼此关系密切。据报道，两者骨骼中的钙化有机物质都含有蛋白质-多糖复合物，在生物钙化的调节中起关键作用。然而，内骨骼骨片的基质蛋白的信息一直缺乏。因此，我们研究了这两个物种的骨片的蛋白质有机基质，以分析存在的蛋白质的序列和功能特性。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析表明，L. crassum和109、83、70、63、41、30和22 kDa的7条带。多趾龙在L. crassum和两条约70和63 kDa的蛋白带。polydactyla产生N-末端氨基酸序列。高碘酸-希夫染色表明，L. crassum和S. polydactyla被糖基化。为了检测钙结合蛋白，通过45 Ca放射自显影在提取物中进行Ca 2+覆盖分析。L. crassum和S.多趾龙被发现具有放射性。对被认为在钙化过程中起重要作用的碳酸酐酶（CA）的测定显示了特定的活性。对新衍生的蛋白质序列进行标准序列分析，包括鉴定与数据库中其他蛋白质的相似性。结果表明，两种植物的蛋白质表达和序列组成存在显著差异。
Two species of alcyonarian corals, Lobophytum crassum and Sinularia polydactyla, are closely related to each other. It is reported that the calcified organic substances in the skeletons of both contain a protein–polysaccharide complex playing a key role in the regulation of biocalcification. However, information on the matrix proteins of endoskeletal sclerite has been lacking. Hence we studied the proteinaceous organic matrices of sclerites for both species, to analyze the sequences and the functional properties of the proteins present. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the preparations showed four bands of proteins with apparent molecular masses of 102, 67, 48, and 37 kDa for L. crassum and seven bands of 109, 83, 70, 63, 41, 30, and 22 kDa for S. polydactyla. A major protein band of about 67 kDa in L. crassum and two bands of proteins of about 70 and 63 kDa in S. polydactyla yielded N-terminal amino acid sequences. Periodic acid-Schiff staining indicated that the 67-kDa protein in L. crassum, and 83- and 63-kDa proteins in S. polydactyla were glycosylated. For detection of calcium binding proteins, a Ca2+ overlay analysis was conducted in the extract via 45Ca autoradiography. The 102- and 67-kDa calcium binding proteins in L. crassum, and the 109- and 63-kDa Ca2+ binding proteins in S. polydactyla were found to be radioactive. An assay for carbonic anhydrase (CA), which is thought to play an important role in the process of calcification, revealed specific activities. Newly derived protein sequences were subjected to standard sequence analysis involving identification of similarities to other proteins in databases. The significantly different protein expressions and compositional analysis of sequences between two species were demonstrated.
DOI：
10.1073/pnas.89.6.2002
发表时间：
1992-03-15
影响因子：
11.1
作者：
BRENDEL, V;BUCHER, P;KARLIN, S
通讯作者：
KARLIN, S