Influence of experimental parameters on the characteristics of poly(lactic acid) nanoparticles prepared by a double emulsion method
Influence of experimental parameters on the characteristics of poly(lactic acid) nanoparticles prepared by a double emulsion method
复制标题
DOI：
10.1016/s0168-3659(97)00106-5
复制
发表时间：
1998-01-02
影响因子：
10.8
通讯作者：
Vigneron, C
中科院分区：
文献类型：
--
作者：
Zambaux, MF;Bonneaux, F;Vigneron, C
以二氯甲烷为溶剂，聚乙烯醇（PVA）或人血清白蛋白（HSA）为表面活性剂，采用复乳法（w/o/w）制备纳米粒。实验参数，如制备温度，溶剂蒸发方法，内部水相体积，表面活性剂浓度和聚合物分子量进行了研究的粒径，zeta电位，残留的表面活性剂的百分比和多分散指数。确定了导致具有明确特征的颗粒的制备参数，例如平均尺寸约为200 nm，多分散指数低于0.1。优化了条件以确保蛋白质包封：冷却温度，短处理时间，足够的内部水相和仔细洗涤。表面活性剂在外水相中的浓度越高，粒子越小，多分散指数越低，表面活性剂的残留量越高。对于PVA或HSA，可以强调方便的表面活性剂浓度与其临界聚集浓度之间的一致性。否则，增加的聚合物分子量导致稍微减小的粒度和更低的多分散性指数。此外，PVA的多层吸附不依赖于聚乳酸的分子量。最后，zeta电位由聚合物分子量和残余PVA引起。(C)1998年Elsevier Science B.V.
Nanoparticles were prepared by the double emulsion method (w/o/w), using methylene chloride as an organic solvent and polyvinyl alcohol (PVA) or human serum albumin (HSA) as a surfactant. Experimental parameters such as the preparation temperature, the solvent evaporation method, the internal aqueous phase volume, the surfactant concentration and the polymer molecular weight were investigated for particle size, the zeta potential, the residual surfactant percentage and the polydispersity index. Preparation parameters leading to particles with well-defined characteristics such as an average size around 200 nm and a polydispersity index lower than 0.1 were identified, The conditions were optimized to ensure protein encapsulation: a cool temperature, a short processing time, a sufficient internal aqueous phase and careful washing. It appeared that the higher the surfactant concentration in the external aqueous phase was, the smaller the particles, the lower the polydispersity index and the higher the residual amount of surfactant were. For PVA or HSA, the agreement between the convenient surfactant concentration and its critical aggregation concentration could be emphasized. Otherwise, an increased polymer molecular weight led both to a slightly decreased particle size and to a lower polydispersity index. Moreover, multilayer adsorption of PVA which does not depend on Poly(lactic-acid) molecular weight was exhibited. Finally, the zeta potential resulted from the polymer molecular weight and the residual PVA. (C) 1998 Elsevier Science B.V.