Two endosymbiotic bacteria, Wolbachia and Arsenophonus, in the brown planthopper Nilaparvata lugens
Two endosymbiotic bacteria, Wolbachia and Arsenophonus, in the brown planthopper Nilaparvata lugens
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褐飞虱 Nilaparvata lugens 中的两种内共生细菌，Wolbachia 和 Arsenophonus
DOI：
10.1007/s13199-013-0256-9
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发表时间：
2013-09-01
期刊：
影响因子：
2.5
通讯作者：
Zhang, Chuan-Xi
中科院分区：
文献类型：
--
作者：
Qu, Lv-Yu;Lou, Yi-Han;Zhang, Chuan-Xi
褐飞虱胞内存在酵母样真菌共生体和内共生细菌，内共生细菌主要包括沃尔巴克氏体和砷菌。本研究在中国和东南亚国家采集的15个褐飞虱种群中均检测到Wolbachia或Arsenophonus。此外，聚合酶链反应（PCR）分析的个人在人口中同时感染沃尔巴克氏体和Arsenophonus表明，每个人都被感染的两种共生细菌之一。实时荧光定量PCR结果表明，这两种内共生菌主要定位于脂肪体的菌细胞中。Wolbachia+和Arsenophonus+褐飞虱种群之间的相互杂交表明，这两种细菌都是母系传播的。结果表明，褐飞虱种群广泛感染Wolbachia和Arsenophonus，这两种细菌可能在每个宿主个体中是排他性的。这一发现将有助于进一步研究内共生细菌的生物学功能，并加深我们对该宿主复杂共生系统的理解。
The brown planthopper Nilaparvata lugens harbors intracellular fungal yeast-like symbionts and endosymbiotic bacteria, with the latter mainly comprising Wolbachia and Arsenophonus. In this study, Wolbachia or Arsenophonus were detected in all 15 brown planthopper populations collected from China and Southeastern Asian countries. Furthermore, Polymerase Chain Reaction (PCR) analysis of the individuals in a population that was infected by both Wolbachia and Arsenophonus showed that each individual was infected by only one of the two symbiotic bacteria. Real-time quantitative PCR showed that both endosymbionts are mainly localized in the mycetocytes of the fat body. Reciprocal crosses between the Wolbachia+ and Arsenophonus+ brown planthopper populations showed that both bacteria were maternally transmitted. Our results showed that the brown planthopper populations are extensively infected by Wolbachia or Arsenophonus, and the two bacteria may be exclusive in each host individual. This finding might be helpful for further studies on the biological functions of the endosymbiotic bacteria and will deepen our understanding of the complicated symbiosis system in this host.