Nonvascular transport distraction osteogenesis in bone formation and regeneration. Is it an accidental phenomenon?
Nonvascular transport distraction osteogenesis in bone formation and regeneration. Is it an accidental phenomenon?
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骨形成和再生中的非血管运输牵张成骨。
DOI：
10.1016/j.jcms.2014.10.004
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Zhou, Nuo
中科院分区：
文献类型：
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作者：
Guo, Peng;Zeng, Jing-Jing;Zhou, Nuo
目的：为探讨非血管输送牵张成骨（NTDO）技术构建犬下颌骨缺损的成骨机制，将60只成年犬随机分为3组，每组20只。建立犬下颌骨NTDO缺损模型。牵张后1、4和12周对动物实施安乐死，收集并固定转移盘和周围组织。结果：1周后，牵张间隙及转移盘周围可见牵张骨再生，新生骨与转移盘形成骨性连接。破骨细胞聚集在转运盘周围，可见骨吸收陷窝形成。牵张4周后，运输盘周围新生骨接近成熟，中间有较厚的硬骨。12周后，新生骨与运输盘完全融合，H&E染色及电镜下难以分辨。结论：NTDO成功修复犬下颌骨缺损，新生骨形成理想，下颌骨生理功能完全恢复。周围组织，包括肌肉骨骼组织、骨膜和其他软组织以及非血管运输盘，共同促进NTDO中的骨再生和新血管形成。(C)2014年欧洲颅颌面外科协会。由爱思唯尔有限公司出版。保留所有权利。
Purpose: To explore the osteogenic mechanism of nonvascular transport distraction osteogenesis (NTDO) by constructing mandibular defects in dogs.Methods: Sixty adult dogs were randomly divided into three groups with 20 dogs in each group. Canine mandibular defect models of NTDO were constructed. Animals were euthanized 1, 4 and 12 weeks after distraction, and the transport disc and surrounding tissue were collected and fixed. Histochemical staining using hematoxylin and eosin (H&E) and electron microscopic observations were used to examine bone regeneration.Results: Distraction bone regeneration was observed in the distraction gap and around the transport disc, and osseous connections had formed between new bone and the transport disc after one week. Osteoclasts gathered around the transport disc, and bone absorption pit formation could be seen. After 4 weeks of distraction, the new bone around the transport disc was close to maturity with thick sclerostin on the middle of the transport disc. After 12 weeks the new bone and the transport disc were fully integrated, and were difficult to distinguish by H&E staining and electron microscopy.Conclusions: Canine mandibular defects were successfully repaired by NTDO resulting in ideal new bone formation and fully recovered mandibular physiological function. The surrounding tissues, including musculoskeletal tissues, the periosteum and other soft tissues and the nonvascular transport disc, together contribute to bone regeneration and neovascularization in NTDO. (C) 2014 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.