Transgenic mice carrying the erythropoietin gene promoter linked to lacZ express the reporter in proximal convoluted tubule cells after hypoxia.
Transgenic mice carrying the erythropoietin gene promoter linked to lacZ express the reporter in proximal convoluted tubule cells after hypoxia.
复制标题
携带与 lacZ 连接的促红细胞生成素基因启动子的转基因小鼠在缺氧后在近曲小管细胞中表达报告基因。
作者：
F. Loya;Y. Yang;H. Lin;E. Goldwasser;M. Albitar
关于促红细胞生成素（Epo）合成在肾细胞中的定位结果一直是矛盾的，可能是间质细胞，也可能是小管细胞。我们将lacZ基因与小鼠Epo基因的一个7 kb的DNA片段融合，该片段包含第一个内含子、第一个外显子的一部分，以及5'侧区一个6 kb的序列。携带该结构的转基因小鼠在近曲小管（PCT）细胞中表现出低水平的lacZ基因特异性表达。无缺氧情况下，肝脏无明显表达。缺氧诱导大量lacZ表达，主要在肾脏PCT细胞中表达，在肝脏中表达较少。然而，贫血诱导lacZ在肾脏和肝脏的表达。这些发现表明，在这些条件下，Epo在小管细胞，特别是PCT细胞中表达。
Results on the localization of erythropoietin (Epo) synthesis in renal cells have been contradictory, implicating either interstitial or tubular cells. We fused the lacZ gene to a 7-kb DNA fragment of the mouse Epo gene encompassing a portion of the first intron, the first exon, and a 6-kb sequence of the 5'-flanking region. Transgenic mice carrying this construct show a low level of specific expression of the lacZ gene in proximal convoluted tubule (PCT) cells. Without hypoxia, no significant expression was detected in the liver. Hypoxia induced a large degree of lacZ expression, mainly in kidney PCT cells and to a lesser degree in liver. However, anemia induced lacZ expression in both kidney and liver. These findings indicate that, under these conditions, Epo is expressed in tubular cells, specifically PCT cells.
DOI：
10.1073/pnas.82.13.4438
发表时间：
1985-01-01
影响因子：
11.1
作者：
BRINSTER, RL;CHEN, HY;PALMITER, RD
通讯作者：
PALMITER, RD