Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans
Automated imaging of neuronal activity in freely behaving Caenorhabditis elegans
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DOI：
10.1016/j.jneumeth.2010.01.011
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发表时间：
2010-03-30
影响因子：
3
通讯作者：
Schafer, William R.
中科院分区：
文献类型：
--
作者：
Ben Arous, Juliette;Tanizawa, Yoshinori;Schafer, William R.
为了了解神经元回路如何控制运动模式，有必要记录自由行为动物的神经元活动。在这里，使用一个新的自动化系统，同时记录的行为和神经元活动在自由移动的秀丽隐杆线虫在标准琼脂平板上，我们表明，自发的逆转，从向前到向后的运动准确地反映了活动的AVA命令interneurons。我们还见证了在标准条件下的蠕虫的自由行为过程中的PLM感觉神经元的自发活动瞬变。我们发现，这些活动瞬态耦合到短的自发向前加速度的蠕虫。(C)2010爱思唯尔有限公司版权所有。
In order to understand how neuronal circuits control locomotory patterns it is necessary to record neuronal activity of freely behaving animals. Here, using a new automated system for simultaneous recording of behavior and neuronal activity in freely moving Caenorhabditis elegans on standard agar plates, we show that spontaneous reversals from forward to backward locomotion reflect precisely the activity of the AVA command interneurons. We also witness spontaneous activity transients in the PLM sensory neurons during free behavior of the worm in standard conditions. We show that these activity transients are coupled to short spontaneous forward accelerations of the worm. (C) 2010 Elsevier B.V. All rights reserved.