Alveolar and lung liquid clearance in the absence of pulmonary blood flow in sheep.
Alveolar and lung liquid clearance in the absence of pulmonary blood flow in sheep.
复制标题
绵羊无肺血流时的肺泡和肺液体清除率。
DOI：
10.1152/jappl.1991.71.5.1679
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Matthay,MA
中科院分区：
文献类型：
--
作者：
Jayr,C;Matthay,MA
在麻醉机械通气绵羊上，研究了肺血流阻断对正常肺内多余液体和蛋白质清除率的影响。为了消除流向一侧肺的肺血流，闭塞左肺动脉。然后将含有125 I标记的白蛋白的自体血清（3 ml/kg）滴入左下叶。测定血流动力学和肺淋巴液，4 h后取肺。以4 h内肺泡蛋白浓度作为肺泡液体清除率的指标。采用重量法测定肺液体总清除率。无肺血流绵羊（33.8 +/- 17.4）和有肺血流绵羊（31.1 +/- 14.6）之间肺泡蛋白浓度较基线增加的百分比相似;因此，肺泡液体清除率未因肺血流缺失而改变。此外，在没有肺血流的情况下，4小时内的肺液体清除率（作为滴注的百分比）为27.1 +/- 3.8（n = 7），这与具有肺血流的对照实验（26.4 +/- 7.1，n = 7）没有显著差异。在没有肺血流的绵羊中，支气管肺吻合口血流为41.5 +/- 21.3 ml/min。为了进一步减少流向肺的血流，左肺动脉和支气管食管动脉均被闭塞，这将流向左肺的支气管肺吻合口血流减少至12.0 +/- 9.3 ml/min。然而，改变肺泡或肺液体清除率。即使左肺血流量如此低，125 I-白蛋白从肺中的清除也没有改变。（250字处删节）
The effect of removing pulmonary blood flow on the clearance of excess liquid and protein from the air spaces and interstitium of the normal lung was studied in anesthetized ventilated sheep. To eliminate pulmonary blood flow to one lung, the left pulmonary artery was occluded. Autologous serum (3 ml/kg) with 125I-labeled albumin was then instilled into the left lower lobe. Hemodynamics and lung lymph were measured, and the lungs were removed after 4 h. Alveolar protein concentration over 4 h was used as the index of alveolar liquid clearance. Total lung liquid clearance was measured by the gravimetric method. The percent increase in alveolar protein concentration over baseline was similar between sheep without pulmonary blood flow (33.8 +/- 17.4) and with pulmonary blood flow (31.1 +/- 14.6); thus, alveolar liquid clearance was not changed by the absence of pulmonary blood flow. Also, in the absence of pulmonary blood flow, lung liquid clearance (as percent of instilled) over 4 h was 27.1 +/- 3.8 (n = 7), which was not significantly different from control experiments with pulmonary blood flow (26.4 +/- 7.1, n = 7). In the sheep without pulmonary blood flow, bronchopulmonary anastomotic flow was 41.5 +/- 21.3 ml/min. To further reduce blood flow to the lung, both the left pulmonary artery and the bronchoesophageal artery were occluded, which reduced bronchopulmonary anastomotic flow to the left lung to 12.0 +/- 9.3 ml/min. This further reduction in blood flow did not, however, change alveolar or lung liquid clearance. Even with this low blood flow to the left lung, the removal of 125I-albumin from the lung was not altered.(ABSTRACT TRUNCATED AT 250 WORDS)