Five Different Types of Putative GnRH Receptor Gene are Expressed in the Brain of Masu Salmon (Oncorhynchus masou)

Five Different Types of Putative GnRH Receptor Gene are Expressed in the Brain of Masu Salmon (Oncorhynchus masou)
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DOI：
10.2108/zsj.20.1117
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发表时间：
2003-09
期刊：
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影响因子：
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通讯作者：
Aya I Jodo;H. Ando;A. Urano
Aya I Jodo;H. Ando;A. Urano
中科院分区：
其他
文献类型：
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作者：
Aya I Jodo;H. Ando;A. Urano

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摘要近年来的研究表明，单个物种中存在多个编码促性腺激素释放激素受体（GnRH-R）的基因。然而，在鲑鱼中，只有一个基因在虹鳟鱼中被发现。因此，我们采用逆转录-聚合酶链反应（RT-PCR）和5′-快速扩增cDNA末端的方法，利用跨膜保守结构域（TM）引物，从马苏鲑脑和垂体中分离了部分cDNA。从一个个体中分离出5个不同的部分cDNA，并命名为msGnRH-R1、R2、R3、R4和R5。它们分为两组，msGnRH-R1，R2，R3和msGnRH-R4，R5。两组共有59-71%的核苷酸序列同一性。系统发育分析表明，前一组与金鱼GnRH-R GfA密切相关，后一组与金鱼GnRH-R GfB密切相关。所有5种msGnRH-R基因在脑中表达，msGnRHR 1、R3和R5在垂体中表达。此外，我们还发现msGnRH-R1在肾脏和卵巢中有mRNA表达，R2在卵巢中有mRNA表达，而msGnRH-R5基因在肌肉、心脏、肾脏和睾丸中广泛表达。成熟和产卵鱼之间的msGnRH-R基因的表达差异，观察在大脑和垂体，除了不断表达的msGnRH-R5。在脑、垂体和肾脏中也表达了一种能够产生由5 TM组成的截短型GnRH-R的msGnRH-R1 mRNA剪接变体。这些结果表明，五种不同类型的推定GnRH-R基因存在，并在马苏鲑鱼脑中表达。
Abstract Recent studies have shown that there are multiple genes encoding gonadotropin-releasing hormone receptor (GnRH-R) in single species. In salmonids, however, only a single gene has been identified in the rainbow trout. We therefore isolated partial cDNAs from the brain and the pituitary of masu salmon Oncorhynchus masou by reverse transcription-polymerase chain reaction and 5′-rapid amplification of cDNA ends, using primers corresponding to conserved transmembrane domains (TMs). Five different partial cDNAs were isolated from an individual and termed as msGnRH-R1, R2, R3, R4 and R5. They are divided into two groups, msGnRH-R1, R2, R3 and msGnRH-R4, R5. Two groups share 59-71% nucleotide sequence identities. Phylogenetic analysis showed that the former group is closely related to the goldfish GnRH-R GfA, and the latter to GfB. All five msGnRH-R genes were expressed in the brain and msGnRHR1, R3 and R5 were expressed in the pituitary. In addition, we found mRNA for msGnRH-R1 in the kidney and ovary, and R2 in the ovary, whereas msGnRH-R5 gene was widely expressed in the muscle, heart, kidney and testis. Differences in the expression of msGnRH-R genes between maturing and spawning fish were observed in the brain and pituitary, except for the constantly expressed msGnRH-R5. A splicing variant of msGnRH-R1 mRNA that is capable of generating a truncated GnRH-R that consists of 5TMs was also expressed in the brain, pituitary and kidney. These results indicate that five different types of putative GnRH-R gene are present and expressed in the brain of masu salmon.