Utility of magnetic resonance imaging and nuclear magnetic resonance-based metabolomics for quantification of inflammatory lung injury
Utility of magnetic resonance imaging and nuclear magnetic resonance-based metabolomics for quantification of inflammatory lung injury
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DOI：
10.1152/ajplung.00515.2007
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发表时间：
2008-07-01
影响因子：
4.9
通讯作者：
Stringer, Kathleen A.
中科院分区：
文献类型：
--
作者：
Serkova, Natalie J.;Van Rheen, Zachary;Stringer, Kathleen A.
磁共振成像（MRI）和代谢核磁共振（NMR）波谱在临床上是可用的，但在实验性肺损伤的量化方面应用很少。对预测性动物模型的需求不断增长，但尚未实现，这可以提高我们对疾病发病机制和治疗干预的理解。核磁共振和核磁共振的融合可以将实验数据的应用扩展到临床环境中。本研究探讨了MRI和代谢NMR检测和量化炎症介导的肺损伤的能力。异氟醚麻醉下，经气管给药IL-1 β和tnf - α诱导雄性B6C3F1小鼠肺部炎症。小鼠在给药后2、4、6和24小时进行MRI检查。在6和24小时采集肺进行代谢核磁共振分析。将IL-1 β + tnf α处理的动物数据与盐水处理的对照小鼠进行比较。与对照组相比，IL-1 β + tnf α处理小鼠的MRI高强度与总肺体积（HTLV）比在2、4和6小时时更高，但在24小时后恢复到对照组水平。HTLV比与组织学和病理终点之间的相关性证实了MRI检测肺部炎症的能力。核磁共振可检测代谢物的主成分分析也显示了一种基于能量代谢的生物标志物被确定的时间模式。这些数据表明，核磁共振和代谢核磁共振在检测和量化炎症介导的肺损伤方面具有实用价值。将这些临床可用的技术整合到肺损伤的实验模型中，可以提高基础科学知识和信息向临床的转化。
Magnetic resonance imaging (MRI) and metabolic nuclear magnetic resonance (NMR) spectroscopy are clinically available but have had little application in the quantification of experimental lung injury. There is a growing and unfulfilled need for predictive animal models that can improve our understanding of disease pathogenesis and therapeutic intervention. Integration of MRI and NMR could extend the application of experimental data into the clinical setting. This study investigated the ability of MRI and metabolic NMR to detect and quantify inflammation-mediated lung injury. Pulmonary inflammation was induced in male B6C3F1 mice by intratracheal administration of IL-1 beta and TNF-alpha under isoflurane anesthesia. Mice underwent MRI at 2, 4, 6, and 24 h after dosing. At 6 and 24 h lungs were harvested for metabolic NMR analysis. Data acquired from IL-1 beta + TNF-alpha-treated animals were compared with saline-treated control mice. The hyperintense-to-total lung volume (HTLV) ratio derived from MRI was higher in IL-1 beta + TNF-alpha-treated mice compared with control at 2, 4, and 6 h but returned to control levels by 24 h. The ability of MRI to detect pulmonary inflammation was confirmed by the association between HTLV ratio and histological and pathological end points. Principal component analysis of NMR-detectable metabolites also showed a temporal pattern for which energy metabolism-based biomarkers were identified. These data demonstrate that both MRI and metabolic NMR have utility in the detection and quantification of inflammation-mediated lung injury. Integration of these clinically available techniques into experimental models of lung injury could improve the translation of basic science knowledge and information to the clinic.