Ultraviolet absorbing compounds provide a rapid response mechanism for UV protection in some reef fish
Ultraviolet absorbing compounds provide a rapid response mechanism for UV protection in some reef fish
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DOI：
10.1016/j.jphotobiol.2016.04.020
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发表时间：
2016-07-01
影响因子：
5.4
通讯作者：
Siebeck, U. E.
中科院分区：
文献类型：
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作者：
Braun, C.;Reef, R.;Siebeck, U. E.
珊瑚鱼的外粘液表面含有紫外线吸收化合物(UVAC)，最突出的是类霉菌素类氨基酸(MAAs)。珊瑚鱼外部粘液中的MAAs被认为是通过防止紫外线辐射(UVR)的破坏作用而起到防晒作用的，然而，关于它们保护作用的直接证据一直缺失。我们测试了UVAC的保护功能，方法是将天然低密度的竹节拟甲藻和高密度的新月海链藻的粘液吸收特性暴露在高剂量的UVR(UVB：13.4W*m(-2)，UVA：6.1W*m(-2))下，并以环丁烷嘧啶二聚体(CPDS)的形式测量由此产生的DNA损伤。对于这两个物种，暴露在1h高UVR脉冲下的UV诱导的DNA损伤量与粘液吸光度呈负相关，粘液吸光度是MAA浓度的替代指标。此外，观察到UVAC浓度在紫外线照射后、捕获后立即和圈养10天后迅速显著增加。在一直保持相对较高紫外线吸收水平的新月毛虫中没有观察到这样的增加。与新月毛虫不同的是，竹黄地鼠使用紫外线通讯，因此必须保持紫外线透明的粘液才能显示其紫外线模式。快速改变粘液透明度的能力可能是在保护免受有害紫外线照射和紫外线通信之间进行权衡的重要适应。(C)2016爱思唯尔B.V.保留所有权利。
The external mucus surface of reef fish contains ultraviolet absorbing compounds (UVAC), most prominently Mycosporine-like Amino Acids (MAAs). MAAs in the external mucus of reef fish are thought to act as sunscreens by preventing the damaging effects of ultraviolet radiation (UVR), however, direct evidence for their protective role has been missing. We tested the protective function of UVAC's by exposing fish with naturally low, Pomacentrus amboinensis, and high, Thalassoma lunare, mucus absorption properties to a high dose of UVR (UVB: 13.4 W * m(-2), UVA: 6.1 W * m(-2)) and measuring the resulting DNA damage in the form of cyclobutane pyrimidine dimers (CPDs). For both species, the amount of UV induced DNA damage sustained following the exposure to a 1 h pulse of high UVR was negatively correlated with mucus absorbance, a proxy for MAA concentration. Furthermore, a rapid and significant increase in UVAC concentration was observed in P. amboinensis following UV exposure, directly after capture and after ten days in captivity. No such increase was observed in T. lunare, which maintained relatively high levels of UV absorbance at all times. P. amboinensis, in contrast to T. lunare, uses UV communication and thus must maintain UV transparent mucus to be able to display its UV patterns. The ability to rapidly alter the transparency of mucus could be an important adaptation in the trade off between protection from harmful UVR and UV communication. (C) 2016 Elsevier B.V. All rights reserved.