MEKC-LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells
MEKC-LIF analysis of rhodamine123 delivered by carbon nanotubes in K562 cells
复制标题
K562 细胞中碳纳米管递送的罗丹明 123 的 MEKC-LIF 分析
DOI：
10.1002/elps.200800737
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发表时间：
2009-06-01
期刊：
影响因子：
2.9
通讯作者：
Le, X. Chris
中科院分区：
文献类型：
--
作者：
Li, Ruibin;Wu, Ren'an;Le, X. Chris
氧化单壁碳纳米管（o - SWNTs）被用作药物载体，通过物理吸附将小分子罗丹明123（Rho123）递送至K562细胞。然而，由于罗丹明123在碳纳米管上的荧光猝灭，细胞内罗丹明123的定量测定较为困难。基于胶束电动毛细管色谱（MEKC）结合激光诱导荧光检测（LIF），开发了一种定量方法用于测定由氧化单壁碳纳米管递送至K562细胞的罗丹明123。在该方法中，运行缓冲液中的十二烷基硫酸钠（SDS）可使吸附在氧化单壁碳纳米管上的罗丹明123解吸，并且由于在毛细管两端施加电势时它们的电泳迁移率不同，罗丹明123可与氧化单壁碳纳米管同时分离。利用这种方法，对多药耐药和多药敏感白血病细胞中罗丹明123的细胞内摄取进行了定量，结果表明氧化单壁碳纳米管可作为潜在的药物载体，通过物理吸附将小分子递送至细胞，同时规避白血病细胞的多药耐药性。
Oxidized single-walled carbon nanotubes (o-SWNTs) were employed as the drug carriers to deliver the small molecules of Rhodamine123 (Rho123) into the K562 cells via physical adsorption. However, due to the fluorescence quenching of Rho123 on carbon nanotubes, the quantitative determination of Rho123 in cells is difficult. Based on the MEKC coupled with LIF detection, a quantitative approach was developed for the determination of Rho123 delivered into K562 cells by o-SWNTs. Where the adsorbed Rho123 on o-SWNTs could be desorbed by SDS in running buffer and be simultaneously separated with o-SWNTs due to the differences of their electrophoretic mobility by applying the electric potential at the both ends of capillary. Using this approach, the intracellular uptakes of Rho123 in multidrug-resistant and multidrug-sensitive leukemia cells were quantified, and the results showed that o-SWNTs could be used as the potential drug carriers to deliver small molecules into cells via the physical adsorption along with the circumventing of multidrug resistance of leukemia cells.