An attempt to reverse cardiac lipotoxicity by aerobic interval training in a high-fat diet- and streptozotocin-induced type 2 diabetes rat model
An attempt to reverse cardiac lipotoxicity by aerobic interval training in a high-fat diet- and streptozotocin-induced type 2 diabetes rat model
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DOI：
10.1186/s13098-019-0436-8
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发表时间：
2019-06-13
影响因子：
4.8
通讯作者：
He, Yuxiu
中科院分区：
文献类型：
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作者：
Cai, Huan;Chen, Shuchun;He, Yuxiu
背景糖尿病（Diabetes mellitus，DM）是心血管疾病的重要危险因素.有氧间歇训练（AIT）已被推荐给患者作为一种非药物策略来管理DM。然而，很少有人知道是否AIT干预在糖尿病发病时将逆转糖尿病心肌病（DCM）的过程。在这项研究中，我们试图评估是否AIT可以逆转DCM的过程，并探讨其潜在的mechanism.Methods50只Wistar大鼠随机分为对照组（CON），DCM组（DCM）和AIT干预组（AIT）。DCM组和AIT组采用高脂饮食和链脲佐菌素（STZ）诱导糖尿病模型。AIT组大鼠进行为期8周的AIT干预。测量空腹血糖（FBG）、血脂和胰岛素水平。苏木精-伊红（HE）染色和油红O染色分别用于鉴定心脏形态和脂质积聚。检测血清BNP水平和心脏BNP mRNA表达，以确保AIT干预的安全性。游离脂肪酸（FFA）和甘油二酯（DAG）的浓度进行了分析，通过酶的方法。采用RT-PCR和Western blotting检测AMPK、p-AMPK、FOXO 1、CD 36和PPAR基因及蛋白表达。
BackgroundDiabetes mellitus (DM) is an important risk factor for cardiovascular disease. Aerobic interval training (AIT) has been recommended to patients as a non-pharmacological strategy to manage DM. However, little is known about whether AIT intervention at the onset of DM will reverse the process of diabetic cardiomyopathy (DCM). In this study, we sought to evaluate whether AIT can reverse the process of DCM and explore the underlying mechanisms.MethodsFifty Wistar rats were randomly divided into a control group (CON), DCM group (DCM) and AIT intervention group (AIT). A high-fat diet and streptozotocin (STZ) were used to induce diabetes in rats in the DCM group and AIT group. Rats in the AIT group were subjected to an 8-week AIT intervention. Fasting blood glucose (FBG), lipid profiles and insulin levels were measured. Haematoxylin and eosin (HE) staining and oil red O staining were used to identify cardiac morphology and lipid accumulation, respectively. Serum BNP levels and cardiac BNP mRNA expression were measured to ensure the safety of the AIT intervention. Free fatty acid (FFA) and diacylglycerol (DAG) concentrations were analysed by enzymatic methods. AMPK, p-AMPK, FOXO1, CD36 and PPAR gene and protein expression were detected by RT-PCR and Western blotting.ResultsAIT intervention significantly reduced rat serum cardiovascular disease risk factors in DCM rats (P