ISOLATION AND CHARACTERIZATION OF RETROVIRUS FROM CELL-LINES OF HUMAN ADULT T-CELL LEUKEMIA AND ITS IMPLICATION IN THE DISEASE
ISOLATION AND CHARACTERIZATION OF RETROVIRUS FROM CELL-LINES OF HUMAN ADULT T-CELL LEUKEMIA AND ITS IMPLICATION IN THE DISEASE
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DOI：
10.1073/pnas.79.6.2031
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
HINUMA, Y
中科院分区：
文献类型：
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作者：
YOSHIDA, M;MIYOSHI, I;HINUMA, Y
在成人T细胞白血病细胞系中明确地发现了一种逆转录病毒(ATLV)，其密度为1.152-1.155 g/cm~3，逆转录酶活性对放线菌素D不敏感，核糖核糖核酸标记，以及相对分子质量为11,000，14,000，17,000，24,000和45,000的特定蛋白。经洗涤剂处理的病毒粒子与含有聚(A)的35S RNA杂交，通过内源性反应制备的互补DNA可被IdUrd[IdUrd]诱导ATL白血病细胞来源的T细胞系诱导，并在ATL来源的T细胞系中检测到整合的ATLV前病毒DNA。5例ATL患者新鲜外周血淋巴细胞中也检出ATLV前病毒DNA，而健康成人外周血中未检出。分子量为42,000的ATLV蛋白至少是ALT白血病患者血清中检测到的一种ATL相关抗原(S)。这些ATLV蛋白和前病毒DNA与ATL密切相关的发现是逆转录病毒ATLV可能参与人类ATL白血病发生的直接证据。
A retrovirus (ATLV [adult T-cell leukemia retrovirus]) was unequivocally demonstrated in human adult T cell leukemia (ATL) cell lines by density (1.152-1.155 g/cm3) in a sucrose gradient, reverse transcriptase activity insensitive to actinomycin D, RNA labeled with [3H]uridine and specific proteins with MW of 11,000, 14,000, 17,000, 24,000 and 45,000. Complementary[c]DNA prepared by endogenous reaction with detergent-treated virions hybridized to 35S RNA containing poly(A), which was inducible by IdUrd [iododeoxyuridine] treatment of a T-cell line derived from leukemic cells of the ATL, and the integrated form of ATLV proviral DNA was detected in T-cell lines derived from ATL. The ATLV proviral DNA was also detected in fresh peripheral lymphocytes from all 5 patients with ATL tested so far, but not in those from healthy adults. ATLV protein of MW 42,000 was at least one of the ATL-associated antigen(s) that were previously detected in ALT-leukemic cells by all sera from patients with ATL. These finding on the close association of ATLV protein and proviral DNA with ATL are direct evidence for the possible involvement of the retrovirus ATLV in leukemogenesis of human ATL.