Infection and Propagation of Astrovirus VA1 in Cell Culture.
Infection and Propagation of Astrovirus VA1 in Cell Culture.
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DOI：
10.1002/cpmc.73
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发表时间：
2019-02-01
影响因子：
--
通讯作者：
Wang, David
中科院分区：
文献类型：
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作者：
Janowski, Andrew B;Wang, David
星状病毒 VA1/HMO-C (VA1) 是乳腺病毒 9 的代表性基因型，乳腺病毒 9 是单链正链 RNA 病毒科星状病毒科的一种。星状病毒传统上被认为是胃肠道病原体，但最近发现它们与人类、牛、水貂、绵羊和猪的神经系统疾病有关。 VA1 是最常从人类脑膜脑炎病例中发现的星状病毒基因型，最近已在细胞培养物中繁殖。 VA1现在可以用作模型系统来研究与星状病毒感染相关的神经系统疾病的发病机制。在本文中，我们描述了两种量化 VA1 复制和传播的基本测定方法，即测量病毒 RNA 的定量逆转录 PCR (qRT-PCR) 和测量感染性病毒颗粒的 50% 组织培养感染剂量 (TCID50) 测定法。 © 2018 John Wiley & Sons, Inc. 版权所有
Astrovirus VA1/HMO-C (VA1) is the representative genotype of mamastrovirus 9, a species of the single-stranded, positive-sense RNA viral family, Astroviridae. Astroviruses have been traditionally considered pathogens of the gastrointestinal tract but they have been recently associated with neurological diseases in humans, cattle, mink, sheep, and pigs. VA1 is the astrovirus genotype most commonly identified from human cases of meningoencephalitis and has been recently propagated in cell culture. VA1 can now be used as a model system to study pathogenesis of the neurological diseases associated with astrovirus infection. In this article, we describe two fundamental assays to quantify replication and propagation of VA1, a quantitative reverse transcription-PCR (qRT-PCR) to measure viral RNA and a 50% tissue culture infectious dose (TCID50 ) assay to measure infectious viral particles. © 2018 by John Wiley & Sons, Inc.