Monoclonal antibody covalently coupled with fatty acid. A reagent for in vitro liposome targeting.
Monoclonal antibody covalently coupled with fatty acid. A reagent for in vitro liposome targeting.
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DOI：
10.1016/s0021-9258(19)70595-x
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发表时间：
1980-09
期刊：
影响因子：
--
通讯作者：
A. Huang;L. Huang;S. Kennel
中科院分区：
文献类型：
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作者：
A. Huang;L. Huang;S. Kennel
小鼠组织相容性抗原的单克隆抗体，H-2k，衍生与棕榈酸使用活化的酯的N-羟基琥珀酰亚胺。通过去污剂透析法，约70%的两亲性抗体可被掺入脂质体中。抗体结合的脂质体的直径为约900 A，并且就每个脂质体的抗体分子数量而言是异质的。这些脂质体显示出对小鼠L-929细胞（H-2k）的特异性结合亲和力，但对A-31细胞（H-2d）没有特异性结合亲和力，而天然脂质体显示出对两种细胞类型都没有可检测的结合。抗H-2k结合脂质体与L-929细胞的特异性结合可通过细胞与过量的游离未衍生化抗H-2k抗体预孵育而被阻断，但不能通过正常小鼠IgG阻断。使用荧光磷脂，含有抗H-2k的脂质体特异性标记混合培养物中的L-929细胞，但不标记A-31细胞。含有正常小鼠IgG的脂质体未显著标记任一细胞类型。这些结果清楚地证明了单克隆抗体用于脂质体靶向的有效性。
Monoclonal antibody to the mouse histocompatibility antigen, H-2k, was derivatized with palmitic acid using an activated ester of N-hydroxysuccinimide. About 70% of the resulting amphipathic antibody could be incorporated into liposomes by a detergent-dialysis method. The antibody-bound liposomes were about 900 A in diameter and were heterogeneous in terms of the number of antibody molecules per liposome. These liposomes showed specific binding affinity to mouse L-929 cells (H-2k), but not to A-31 cells (H-2d), whereas native liposomes showed no detectable binding to either cell type. The specific binding of anti-H-2k-bound liposomes to L-929 cells could be blocked by a preincubation of cells with an excess of free, underivatized anti-H-2k antibody but not by normal mouse IgG. Using a fluorescent phospholipid, liposomes containing anti-H-2k specifically labeled L-929 cells but not A-31 cells in a mixed culture. Liposomes containing normal mouse IgG did not significantly label either cell type. These results clearly demonstrated the effectiveness of the monoclonal antibody for liposome targeting.