Osmoresistant yeast Zygosaccharomyces rouxii: the two most studied wild‐type strains (ATCC 2623 and ATCC 42981) differ in osmotolerance and glycerol metabolism
Osmoresistant yeast Zygosaccharomyces rouxii: the two most studied wild‐type strains (ATCC 2623 and ATCC 42981) differ in osmotolerance and glycerol metabolism
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DOI：
10.1002/yea.1470
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发表时间：
2007-03
期刊：
影响因子：
2.6
通讯作者：
L. Přibylová;J. de Montigny;H. Sychrová
中科院分区：
文献类型：
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作者：
L. Přibylová;J. de Montigny;H. Sychrová
鲁氏接合酵母（Zygosaccharomycesrouxii）对渗透胁迫具有高耐受性，这被认为是由一组特定基因引起的。相对较少的Z。rouxii基因在酿酒酵母中均具有同源性，没有一个与Z. rouxii特有的大多数已知的Z rouxii基因分离自两种野生型菌株ATCC 2623和ATCC 42981。在这项研究中，我们比较了这两个菌株的一些形态，生理和基因组特性。它们在耐盐性、甘油同化能力和核型方面存在重要差异，在细胞形态上也存在细微差异。ATCC 42981菌株显示出更高的耐盐性、更高的甘油产量，并且与ATCC 2623不同，能够同化甘油。在渗透胁迫条件下，两种玉米的甘油产量均低于对照。rouxii菌株的感染率远低于S.酿酒酵母S288c培养，这表明存在一个系统，有效地保留甘油内Z。rouxii细胞核型分析显示，ATCC 42981细胞含有更多的染色体，并具有比ATCC 2623细胞更大的基因组大小。版权所有© 2007约翰威利父子有限公司。
The yeast Zygosaccharomyces rouxii is known for its high tolerance to osmotic stress, which is thought to be caused by sets of specific genes. Relatively few Z. rouxii genes have been identified so far, all of them having homologues in Saccharomyces cerevisiae; none of them was Z. rouxii‐specific. Most of the known Z. rouxii genes were isolated from two wild‐type strains, ATCC 2623 and ATCC 42981. In this study, we compared these two strains with regard to some of their morphological, physiological and genomic properties. Important differences were found in their salt tolerance and assimilation of glycerol and karyotype; slight differences were also present in their cell morphology. The ATCC 42981 strain showed a higher resistance to salts, higher glycerol production and, unlike ATCC 2623, was able to assimilate glycerol. Under conditions of osmotic stress, the glycerol production in both Z. rouxii strains was much lower than in a S. cerevisiae S288c culture, which suggested the presence of a system that efficiently retains glycerol inside Z. rouxii cells. The karyotype analysis revealed that ATCC 42981 cells contain more chromosomes and have a bigger genome size than those of ATCC 2623. Copyright © 2007 John Wiley & Sons, Ltd.