UTRome.org:: a platform for 3UTR biology in C-elegans
UTRome.org:: a platform for 3UTR biology in C-elegans
复制标题
DOI：
10.1093/nar/gkm946
复制
发表时间：
2008-01-01
影响因子：
14.9
通讯作者：
Gunsalus, Kristin C.
中科院分区：
文献类型：
--
作者：
Mangone, Marco;MacMenamin, Philip;Gunsalus, Kristin C.
三原基非翻译区(3UTRs)被广泛认为是mRNAs重要的转录后调节区。RNA结合蛋白和小的非编码RNA如microRNAs(MiRNAs)与3UTRs内的功能元件结合，影响mRNA的稳定性、翻译和定位。这些相互作用在发育、新陈代谢和疾病中发挥着许多重要作用。然而，即使在注释最充分的后生动物基因组中，3UTRs及其功能元件也没有得到很好的定义。因此，对3UTRs及其功能元件进行全面和准确的全基因组注释至关重要。我们已经开发了一个开放访问的数据库，可以在http://www.UTRome.org，上获得，以便在特征良好、实验上易于处理的模式系统秀丽线虫中为3UTR生物学提供丰富和全面的资源。UTRome.org结合了来自公共储存库的数据和我们正在进行的一项大规模工作，以表征线虫中的3UTRs及其功能元件，包括3UTR序列、图形显示、预测和验证的功能元件、二级结构预测以及我们克隆过程中的详细数据。UTRome.org将随着时间的推移而大幅增长，涵盖大多数基因的单个3UTR异构体、新的和修订的功能元件，以及可获得的关于3UTR功能的体内数据。因此，罗马大学的数据库是更好地了解3UTRs生物学的有力工具。
Three-prime untranslated regions (3UTRs) are widely recognized as important post-transcriptional regulatory regions of mRNAs. RNA-binding proteins and small non-coding RNAs such as microRNAs (miRNAs) bind to functional elements within 3UTRs to influence mRNA stability, translation and localization. These interactions play many important roles in development, metabolism and disease. However, even in the most well-annotated metazoan genomes, 3UTRs and their functional elements are not well defined. Comprehensive and accurate genome-wide annotation of 3UTRs and their functional elements is thus critical. We have developed an open-access database, available at http://www.UTRome.org, to provide a rich and comprehensive resource for 3UTR biology in the well-characterized, experimentally tractable model system Caenorhabditis elegans. UTRome.org combines data from public repositories and a large-scale effort we are undertaking to characterize 3UTRs and their functional elements in C. elegans, including 3UTR sequences, graphical displays, predicted and validated functional elements, secondary structure predictions and detailed data from our cloning pipeline. UTRome.org will grow substantially over time to encompass individual 3UTR isoforms for the majority of genes, new and revised functional elements, and in vivo data on 3UTR function as they become available. The UTRome database thus represents a powerful tool to better understand the biology of 3UTRs.