ESTABLISHMENT OF NEURONAL CONNECTIVITY DURING DEVELOPMENT OF THE DROSOPHILA LARVAL VISUAL-SYSTEM
ESTABLISHMENT OF NEURONAL CONNECTIVITY DURING DEVELOPMENT OF THE DROSOPHILA LARVAL VISUAL-SYSTEM
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DOI：
10.1002/neu.480280305
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发表时间：
1995-11-01
期刊：
影响因子：
--
通讯作者：
STELLER, H
中科院分区：
文献类型：
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作者：
CAMPOS, AR;LEE, KJ;STELLER, H
我们使用共聚焦显微镜结合特异性抗体和增强子陷阱菌株，在一个简单的模型系统，果蝇的幼虫视觉系统，以调查特定的神经元连接的发展。我们发现，从幼虫感光神经元到其中枢神经系统靶点的轴突投射的建立涉及一系列离散的步骤。在胚胎发育过程中，幼虫视神经接触几种不同的细胞类型，包括视叶先锋（OLP）神经元和一些胶质细胞，我们证明，OLP神经元存在于玻璃（gl）突变胚胎中，其中幼虫视神经未能发育，表明它们不依赖于与幼虫视神经的相互作用，远分化和适当的轴突投射，OLP无法在断开（迪斯科）突变胚胎中正确分化，其中未形成幼虫视神经及其大脑中目标之间的适当连接。disco基因在OLP中表达，因此可以自主地指导这些细胞的分化。总之，我们的研究结果表明，OLP作为一个中间目标所需的建立正常的视神经投射和连接。(C)John Wiley & Sons，Inc.
We used confocal microscopy in conjunction with specific antibodies and enhancer trap strains to investigate the development of specific neuronal connections in a simple model system, the larval visual system of Drosophila. We find that the establishment of axonal projections from the larval photoreceptor neurons to their central nervous system targets involves a series of discrete steps. During embryogenesis, the larval optic nerve contacts several different cell types, including optic lobe pioneer (OLP) neurons and a number of glial cells, We demonstrate that OLP neurons are present and project normally in glass (gl) mutant embryos in which the larval optic nerve fails to develop, suggesting that they do not depend on interactions with the larval optic nerve far differentiation and proper axonal projection, The OLPs fail to differentiate properly in disconnected (disco) mutant embryos, where appropriate connections between the larval optic nerve and its targets in the brain are not formed. The disco gene is expressed in the OLPs and may therefore act autonomously to direct the differentiation of these cells. Taken together, our results suggest that the OLPs act as an intermediate target required for the establishment of normal optic nerve projection and connectivity. (C) 1995 John Wiley & Sons, Inc.