Germ cells are required to maintain a stable sexual phenotype in adult zebrafish
Germ cells are required to maintain a stable sexual phenotype in adult zebrafish
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DOI：
10.1016/j.ydbio.2013.01.016
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发表时间：
2013-04-01
影响因子：
2.7
通讯作者：
Draper, Bruce W.
中科院分区：
文献类型：
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作者：
Dranow, Daniel B.;Tucker, Richard P.;Draper, Bruce W.
斑马鱼的性别不是由主要的染色体位点决定的，而是依赖于一种受生殖细胞来源的信号影响的机制，因为缺乏生殖细胞或特别是卵母细胞的动物发育为表型雄性。这些数据表明，在初级性别决定，卵母细胞来源的信号作用于双能体细胞性腺，以促进女性特定的程序。然而，目前尚不清楚生殖细胞是否仅在初级性别决定窗口期需要，还是在整个成年期都需要生殖细胞来维持雌性性表型。在这里，我们表明，虽然野生型斑马鱼不切换性别作为成年人，生殖细胞耗尽的成年女性很容易转换为男性表型。值得注意的是，当卵母细胞被耗尽，但生殖系干细胞仍然存在，成年女性性回复为产精子的男性，表明生殖细胞来源的信号作用于体细胞性腺，通过抑制男性特异性基因表达直接或间接促进女性发育。这些结果也证实了来自体细胞性腺的信号反过来确保产生性别适当的配子。(C)2013 Elsevier Inc. All rights reserved.
Sex in zebrafish is not determined by a major chromosomal locus, but instead relies on a mechanism that is influenced by a germ cell-derived signal, as animals that lack germ cells, or specifically oocytes, develop as phenotypic males. These data suggest that during primary sex determination, an oocyte-derived signal acts on the bipotential somatic gonad to promote the female-specific program. However, it is not known if germ cells are required only during the primary sex-determining window, or if they are required throughout adult life to maintain the female sexual phenotype. Here, we show that while wild-type zebrafish do not switch sex as adults, germ cell-depleted adult females readily convert to a male phenotype. Notably, when oocytes are depleted, but germline stem cells remain, adult females sex-revert to sperm-producing males, indicating that a germ cell-derived signal acts on the somatic gonad to promote female development directly or indirectly by repressing male-specific gene expression. These results also confirm that signals from the somatic gonad in turn ensure that the sex appropriate gamete is produced. (C) 2013 Elsevier Inc. All rights reserved.