IS3 CAN FUNCTION AS A MOBILE PROMOTER IN ESCHERICHIA-COLI
IS3 CAN FUNCTION AS A MOBILE PROMOTER IN ESCHERICHIA-COLI
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DOI：
10.1093/nar/10.19.5935
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
GLANSDORFF, N
中科院分区：
文献类型：
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作者：
CHARLIER, D;PIETTE, J;GLANSDORFF, N
我们已经表明，以前(3)。coliargEgene可以通过在靠近基因5 '端的II取向插入IS3元件来开启。在这里，我们证明了这种效应是由于位于IS3上的一个向外启动子的存在。通过DNA测序确定了IS3的确切插入位置。利用内转录RNA中的S1核酸酶定位技术，我们在IS3上定位了负责arge转录的启动子，该启动子位于该元件反向重复的区域之外。因此，IS3可被视为移动促进剂。
We had shown previously (3) that theE.coliargEgene could be turned-on by an IS3 element inserted in orientation II near the 5′ end of the gene. Here we show that this effect is due to the presence of an outward promoter located on IS3. The exact site of insertion of IS3 was determined by DNA sequencing. Using the S1 nuclease mapping technique withinvivotranscribed RNA we located the promoter responsible forargEtranscription on IS3 itself outside the region involved in the inverted repeats of this element. IS3 may therefore be considered as a mobile promoter.